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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABE1039 Sigma-AldrichAnti-Nup96 Antibody, clone 4H5

Anti-Nup96 Antibody, clone 4H5 is an antibody against Nup96 for use in Western Blotting and Immunocytochemistry.

Anti-Nup96 Antibody, clone 4H5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
技术信息

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: Nup98 UniProt Number: Q6PFD9

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	WB, ICC	R	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE1039
Description	Anti-Nup96 Antibody, clone 4H5
Alternate Names	Nuclear pore complex protein Nup98-Nup96 Nuclear pore complex protein Nup98 98 kDa nucleoporin Nucleoporin Nup98 Nup98 Nuclear pore complex protein Nup96 96 kDa nucleoporin Nucleoporin Nup96 Nup96 Nup96
Background Information	The murine gene Nup98 (Gene ID 269966; also known as AI849286, 4732457F17) encodes the 1816-amino acid nuclear pore complex protein Nup98-Nup96 (UniProt Q6PFD9), which is posttranslationally autocleaved between aa 800 and 801 to form Nup98 (aa 1-800) and Nup96 (aa 801-1816). The nuclear pore complex (NPC) acts as a passageway for nucleocytoplasmic transport. The core structure of the NPC is maintained by the Nup93-205 complex sandwiched between the supporting structures of the Nup107-160 complex on the nuclear and cytoplasmic faces of the pore. Nup96 is a component of the Nup107-160 complex, the largest subunit of the NPC. Nup96 knockout is reported to be fatal in mice and Nup96(+/-) mice with low levels of Nup96 display impaired immune response and are highly susceptible to viral infection.

References

Product Information
Format	Purified
Presentation	Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Nup96 Antibody, clone 4H5 is an antibody against Nup96 for use in Western Blotting and Immunocytochemistry.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Western Blotting Analysis: A representative lot detected Nup96 in NIH/3T3 cells (Mizuguchi, C., et al. (2010). Hybridoma. 29(6):551-553). Immunocytochemistry Analysis: A representative lot detected Nup96 in NIH/3T3 cells (Mizuguchi, C., et al. (2010). Hybridoma. 29(6):551-553).

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to mouse Nup96 near the N-terminus.
Epitope	Near N-terminus.
Clone	4H5
Concentration	Please refer to lot specific datasheet.
Host	Rat
Specificity	Reacts with Nup98-Nup96 precursor (isoforms 1, 2, and 6), nucleoporin Nup96 (isoform 5), but not nucleoporin Nup98 (isoform 3).
Isotype	IgG2aκ
Species Reactivity	Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001274093
Gene Symbol	Nup98
Purification Method	Protein G Purified
UniProt Number	Q6PFD9
Molecular Weight	~120 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in NIH/3T3 cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected Nup96 in 10 µg of NIH/3T3 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABE1039	04055977170689

Documentation

Anti-Nup96 Antibody, clone 4H5 MSDS

职位
物料安全数据表 (MSDS)

Anti-Nup96 Antibody, clone 4H5 分析证书

标题	批号
Anti-Nup96, clone 4H5 - 3327585	3327585
Anti-Nup96, clone 4H5 - 3931393	3931393
Anti-Nup96, clone 4H5 - 4110918	4110918
Anti-Nup96, clone 4H5 -Q2573209	Q2573209

技术信息

标题
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White Paper: Further considerations of antibody validation and usage.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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