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MAB1286-I-25UG Anti-Nuclear Spliceosomes Antibody, clone 780-3

Anti-Nuclear Spliceosomes, clone 780-3, Cat. No. MAB1286-I, is a mouse monoclonal antibody that detects Nuclear spliceosomes and is tested for use in Electron Microscopy, Flow Cytometry, Immunofluorescence, and Western Blotting.

MAB1286-I-25UG
25 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMAB1286-I-25UG
DescriptionAnti-Nuclear Spliceosomes Antibody, clone 780-3
Alternate Names
  • Spliceosome
Background InformationMost eukaryotic genes are expressed as pre-mRNAs that are converted to mRNA by splicing. In this process noncoding sequences (introns) are removed, and coding sequences (exons) are ligated together. Some exons are known to be constitutively spliced and are present in every mRNA produced from a given pre-mRNA. However, many are alternatively spliced to generate variable forms of mRNA from a single pre-mRNA species. Nuclear pre-mRNA splicing is catalyzed by the spliceosome that is a multi-megadalton ribonucleoprotein (RNP) complex. It removes introns from nuclear pre-mRNA. The conformation and composition of spliceosome is shown to be dynamic, which is critical for its high degree of accuracy and flexibility. In most eukaryotes two unique spliceosomes have been described: the U2 -dependent spliceosome that catalyzes the removal of U2-type of introns and the U12-dependent spliceosome that is present only in a subset of eukaryotes and splices U12-type of intron. Introns are removed by two consecutive transesterification reactions. In the first reaction the 2 OH group of the branch adenosine of the intron carries out a nucleophilic attack on the 5 ss, which results in cleavage at this site and ligation of the 5 end of the intron to the branch adenosine, forming a lariat structure. In the second reaction, the 3 ss is attacked by the 3 OH group of the 5 exon, which leads to the ligation of the 5 and 3 exons forming the mRNA, and release of the intron. (Ref.: Matera, AG., and Wang, Z. (2014). Nat. Rev. Mol. Cell Biol. 15(2); 108-121; Will, CL., and Luhrmann, R. (2011). Cold Spring Harb. Perspect. Biol. 3(7); a003707; Patel, AA., and Steitz, JA. (2003). Nat. Rev. Mol. Cell Biol. 4(12); 960-970).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-Nuclear Spliceosomes, clone 780-3, Cat. No. MAB1286-I, is a mouse monoclonal antibody that detects Nuclear spliceosomes and is tested for use in Electron Microscopy, Flow Cytometry, Immunofluorescence, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Western Blotting
  • Electron Microscopy
  • Immunofluorescence
Application NotesTested Applications

Flow Cytometry Analysis: A representative lot detected Nuclear Spliceosomes in Flow Cytometry applications (Clevenger, C.V., et al. (1985). Cytometry. 6(3): 208-14; Bauer, K.D., et al. (1986). Cancer Res. 46(5): 2428-34; Clevenger, C.V., et al. (1987). Cytometry. 8(3): 280-6).

Immunofluorescence Analysis: A representative lot detected Nuclear Spliceosomes in Immunofluorescence applications (Clevenger, C.V., et al. (1984). J Histochem Cytochem. 32(7): 757-65; Clevenger, C.V., et al. (1985). Cytometry. 6(3): 208-14; Bauer, K.D., et al. (1986). Cancer Res. 46(5): 2428-34; Clevenger, C.V., et al. (1987). Cytometry. 8(3):280-6; Clevenger, C.V., et al. (1987). Cytometry. 8(3): 280-6).

Western Blotting Analysis: A representative lot detected Nuclear Spliceosomes in Western Blotting applications (Clevenger, C.V., et al. (1987). Cytometry. 8(3): 280-6; Kumar, P., et al. (2012). PLoS One. 7(8): e42712).

Electron Microscopy: A representative lot detected Nuclear Spliceosomes in Electron Microscopy applications (Clevenger, C.V., et al. (1984). J Histochem Cytochem. 32(7): 757-65; Bauer, K.D., et al. (1986). Cancer Res. 46(5): 2428-34; Clevenger, C.V., et al. (1987). Cytometry. 8(3): 280-6).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenNuclear preparation from U266 human myeloma cells.
EpitopeUnknown
Clone780-3
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 780-3 is a mouse monoclonal antibody that detects human Nuclear spliceosomes.
IsotypeIgMκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Purification MethodLigatrap
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in permeabilized HeLa cells.

Flow Cytometry Analysis: 2 µg of this antibody detected Nuclear Spliceosomes in one million permeabilized HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MAB1286-I-25UG 04065267780566