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MABN845 Anti-Neurophysin 2/NP-AVP Antibody, clone PS 41

MABN845
100 μg  
Purchase on Sigma-Aldrich

特卖

概述

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWB, IHC, RIA, EM, IP, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN845
DescriptionAnti-Neurophysin 2/NP-AVP Antibody, clone PS 41
Alternate Names
  • Vasopressin-neurophysin 2-copeptin
  • AVP-NPII
  • Arg-vasopressin
  • Arginine-vasopressin
  • Neurophysin 2
  • Neurophysin-I
  • Copeptin
  • Neurophysin 2/NP-AVP
Background InformationThe neurophysin 1 and 2 (NPI and NPII) are carriers of peptide hormone oxytocin (OT) and vasopressin (AVP), respectively. Each NP and its associated hormone peptide are synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or AVP-NPII (UniProt P01186) encoded by the Avp gene in rat species. Post-translational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI) or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII). OT is a potent hormone and neurotransmitter secreted within the pituitary gland. OT exerts its biological activity through its receptor OXTR (also known as OTR). OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders. AVP functions primarily as an anti-diuretic and regulates the concentration of water, glucose, and salts in blood. AVP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R).
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Neurophysin 2/NP-AVP Antibody, clone PS 41 is validated for use in Western Blotting and Immunohistochemistry, Radioimmunoassay, Electron Microscopy, Immunoprecipitation and ELISA for the detection of Neurophysin 2/NP-AVP.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Radioimmunoassay
  • Electron Microscopy
  • Immunoprecipitation
  • ELISA
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Neurophysin 2/NP-AVP in rat pituitary tissue.
Immunohistochemistry Analysis: A representative lot detected Neurophysin 2/NP-AVP immunoreactivity in the hypothalamus of developing rats as early as embryonic day (E16) using coronal vibratome sections. (Witnall, M.H., et al.(1985). J Neurosci. 5(1):98-109).
Radioimmunoassay Analysis: Target reactivity of a representative lot was confirmed by RIA using plates coated with purified rat Neurophysin/NP and 125I-labelled secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Electron Microscopy Analysis: A representative lot immunostained granulated neurosecretory vesicles (NSVs) in all axons, Herring bodies, and terminals by EM using rat posterior pituitary LR White sections (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Immunoprecipitation Analysis: A representative lot immunoprecipitated radiolabelled neurophysin 2, but not neurophysin 1 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
ELISA Analysis: A representative lot detected neurophysin 2 immunoreactivity in rat neurophysin preparations by non-sandwich ELISA using either 125I-labelled or HRP-conjugated secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
Biological Information
ImmunogenKLH-conjugated acid soluble extract of rat (Sprague-Dawley) intermediate lobes.
EpitopeNeurophysin 2
ClonePS 41
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone PS 41 reacts with Vasopressin-neurophysin 2-copeptin precursor, neurophysin 2, but not Arg-vasopressin, copeptin, oxytocin, or neurophysin 1.
IsotypeIgG2bκ
Species Reactivity
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Avp
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~15 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat pituitary tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Neurophysin 2/NP-AVP in 10 µg of rat pituitary tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Global Trade Item Number
产品目录编号 GTIN
MABN845 04055977294187