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Recognizes neuropeptide Y in cortex, caudate, putamen, and paraventricular nucleus.
Catalogue Number
PC223L
Brand Family
Calbiochem®
Application Data
Detection of rat neuropeptide Y by immunohistochemistry. Sample: Rat paraventricular nucleus, cortex, and caudate putamen. Primary antibody: Anti-Neuropeptide Y Rabbit pAb (Cat. No. PC223L) (1:10,000). Detection: fluorescence.
Frozen Sections (1:1000-1:2000 for free-floating, PAP detection)
Application Comments
Antibody specificity was examined in the rat cortex, caudate putamen, and paraventricular nucleus. Staining is completely eliminated by pretreatment of antibody with neuropeptide Y (10-6M). Preabsorption with avian pancreatic polypeptide, β-endorphin, cholecystokinin, peptide YY, somatostatin, or vasoactive intestinal peptide resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
porcine neuropeptide Y
Immunogen
Porcine
Host
Rabbit
Isotype
IgG
Species Reactivity
Amphibian
Avian
Feline
Fish
Human
Newt
Rat
Antibody Type
Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-October-2007 RFH
Application
Frozen Sections (1:1000-1:2000 for free-floating, PAP detection)
Application Data
Detection of rat neuropeptide Y by immunohistochemistry. Sample: Rat paraventricular nucleus, cortex, and caudate putamen. Primary antibody: Anti-Neuropeptide Y Rabbit pAb (Cat. No. PC223L) (1:10,000). Detection: fluorescence.
Description
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes neuropeptide Y.
Background
Neurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. Neuropeptide Y (NPY) is a 36 amino acid peptide belonging to the pancreatic polypeptide family of neuroendocrine hormones. NPY is abundantly expressed in neurons within the central and peripheral nervous systems, including the hypothalamus and pituitary. NPY is a potent stimulator of food intake, and may also influence other growth hormones. A family of neuropeptide Y receptors (Y1-Y5) have been identified which recognize both neuropeptide Y and the related Peptide YY. The immunolocalization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
Host
Rabbit
Immunogen species
Porcine
Immunogen
porcine neuropeptide Y
Isotype
IgG
Species
amphibian, avian, feline, fish, human, newt, rat
Positive control
Paraventricular nucleus, cortex, or caudate putamen
Form
Lyophilized
Formulation
Undiluted serum.
Preservative
≤0.1% sodium azide
Comments
Antibody specificity was examined in the rat cortex, caudate putamen, and paraventricular nucleus. Staining is completely eliminated by pretreatment of antibody with neuropeptide Y (10-6M). Preabsorption with avian pancreatic polypeptide, β-endorphin, cholecystokinin, peptide YY, somatostatin, or vasoactive intestinal peptide resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
