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MABF1986-25UL Anti-NKR-P1G Antibody, clone ADA3

Anti-NKR-P1G, clone ADA3, Cat. No. MABF1986 is an Armenian hamster monoclonal antibody that detects NKG-P1G and has beent tested for use in Flow Cytometry and Immunoprecipitation.

Anti-NKR-P1G Antibody, clone ADA3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Klrb1, Klrb1d, Klrb1g Klrb6, Nkrp1e, Nkrp1g UniProt Number: Q0ZUP0
MABF1986-25UL
25 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
RFC, IPAHmPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF1986-25UL
DescriptionAnti-NKR-P1G Antibody, clone ADA3
Alternate Names
  • Killer cell lectin-like receptor subfamily B member 1
  • Immunoreceptor NKR-P1E
  • Killer cell lectin-like receptor subfamily B member 1G
  • Natural killer cell surface protein NKR-P1G
  • Natural killer lectin-like receptor 1E
Background InformationKiller cell lectin-like receptor subfamily B member 1 (UniProt: Q0ZUP0; also known as Immunoreceptor NKR-P1E, Killer cell lectin-like receptor subfamily B member 1G, Natural killer cell surface protein NKR-P1G, Natural killer lectin-like receptor 1E) is encoded by the Klrb1 (also known as Klrb1d, Klrb1g, Klrb6, Nkrp1e, Nkrp1g) gene (Gene ID: 689817) in rat. NKR-P1G is a single-pass type II membrane protein with a cytoplasmic domain (aa 1-42), a helical domain (aa 43-63), and an extracellular domain (aa 64-214). NKR-P1G is expressed by subsets of lymphocytes with highest prevalence in gut and liver mucosa. The number of NKR-P1G+ cells are shown to increase with age and its expression level is modulated by cytokines, with an increase induced by inflammatory cytokines. It is shown to be mainly expressed by Ly49s3+ cells. It is reported to inhibit cytotoxicity and calcium responses in IL-2-activated NK cells. NKR-P1G is a potent inhibitory receptor for a panel of Clr ligands. The intracellular domain of NKR-P1G contains an immunoreceptor tyrosine-based inhibitory motif that is phosphorylated by Src-kinases upon activation. This allows recruitment of phosphotyrosine phosphatases, which in turn negatively regulates the signaling cascades that is essential for cytotoxicity and cytokine production. (Ref.: Kveberg, L et al (2015). Eur J Immunol. 45(2); 501-512).
References
Product Information
FormatPurified
PresentationPurified Armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-NKR-P1G, clone ADA3, Cat. No. MABF1986 is an Armenian hamster monoclonal antibody that detects NKG-P1G and has beent tested for use in Flow Cytometry and Immunoprecipitation.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot detected NKR-PIG in Immunoprecipitation applications (Kveberg, L., et. al. (2015). Eur J Immunol. 45(2):501-12).

Flow Cytometry Analysis: A representative lot detected NKR-PIG in Flow Cytometry applications (Kveberg, L., et. al. (2015). Eur J Immunol. 45(2):501-12).
Biological Information
ImmunogenChinese hamster ovary (CHO) cells stably transfected with FLAG-tagged extracellular portion of NKR-P1G.
ConcentrationPlease refer to lot specific datasheet.
HostArmenian Hamster
SpecificityClone ADA3 is an Armenian hamster monoclonal antibody that detects Killer cell lectin-like receptor subfamily B member 1 (NKR-P1G) in rat. It targets an epitope within the extracellular domain.
IsotypeIgG
Species Reactivity
  • Rat
Species Reactivity NoteRat.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Klrb1
  • Klrb1d
  • Klrb1g Klrb6
  • Nkrp1e
  • Nkrp1g
Purification MethodProtein G purified
UniProt Number
Molecular Weight24 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Ltk (mouse fibroblast) cells stably transfected with FLAG-tagged rat NKR-P1G.

Flow Cytometry Analysis: 1 µg of this antibody detected NKR-P1G in Ltk (mouse fibroblast) cells stably transfected with FLAG-tagged rat NKR-P1G.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABF1986-25UL 04054839476860