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MABE956 Anti-NFE2-related factor 1/Nrf1 Antibody, clone 17

MABE956
100 µg  
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE956
DescriptionAnti-NFE2-related factor 1/Nrf1 Antibody, clone 17
Alternate Names
  • Nuclear factor erythroid 2-related factor 1
  • NF-E2-related factor 1
  • NFE2-related factor 1
  • Nuclear factor, erythroid derived 2, like 1
Background InformationNuclear factor erythroid 2-related factor 1 (NFE2-related factor 1/Nrf1), also known as NF-E2-related factor 1, NFE2-related factor 1, Nuclear factor erythroid derived 2 like 1, and encoded by the gene name Nfe2l1 and Nrf1, is a member of the bZIP family and the CNC subfamily. Isoform 3 of Nrf1 interacts with the extended kappa 3 site of the TNF alpha promoter after Fc gamma RIII stimulation and participates in the induction of this cytokine. Studies have suggested that isoform 1 of Nrf1 is either inactive or represses the transcriptional activation. Isoform 2 of Nrf1 has been shown to activate the expression of antioxidant response element (ARE) containing reporters and genes in cells, and may play a role in oxidative stress response. Other studies have suggested that isoform 1 also activates ARE-driven genes. Levels of Nrf1 isoform 2 in white fat, lung and liver are increased compared to isoform 1 (at protein level). Isoform 1 and isoform 2 of Nrf1 are widely expressed including kidney, brown fat, white fat, large intestine, small intestine, stomach, lung, brain and liver. Isoform 2 levels are elevated in brown fat and brain, but are reduced in liver compared to isoform 1 levels. Isoform 1 and isoform 2 are also expressed in mouse embryonic fibroblasts (MEF).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-NFE2-related factor 1/Nrf1 antibody is validated for use in Western Blotting for the detection of NFE2-related factor 1/Nrf1.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected NFE2-related factor 1/Nrf1 in MEF cells stimulated with MG132 (Tsuchiya, Y., et al. (2011) Mole. Cell Biol. 31(22):4500-4512).
Biological Information
ImmunogenHis-tagged recombinant protein corresponding to mouse NFE2-related factor 1/Nrf1.
Clone17
ConcentrationPlease refer to lot specific datasheet.
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Nfe2l1
  • Nrf1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~85 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MG132 treated NIH-3T3 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected NFE2-related factor 1/Nrf1 in 10 µg of MG132 treated NIH-3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABE956 04055977116984

Documentation

Anti-NFE2-related factor 1/Nrf1 Antibody, clone 17 MSDS

职位

物料安全数据表 (MSDS) 

Anti-NFE2-related factor 1/Nrf1 Antibody, clone 17 分析证书

标题批号
Anti-NFE2-related factor 1/Nrf1, clone 17 -Q2485588 Q2485588
Anti-c-Myc, clone 9E10 -2708956 2708956

参考

参考概述公共医疗ID
Dual regulation of the transcriptional activity of Nrf1 by β-TrCP- and Hrd1-dependent degradation mechanisms.
Tsuchiya, Y; Morita, T; Kim, M; Iemura, S; Natsume, T; Yamamoto, M; Kobayashi, A
Molecular and cellular biology  31  4500-12  2011

显示摘要
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