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MAB13424 Anti-MMP-13 Antibody, clone VIIIA2

MAB13424
100 µg  
Purchase on Sigma-Aldrich

特卖

概述

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RbIHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB13424
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-MMP-13 Antibody, clone VIIIA2
Alternate Names
  • Collagenase-3
Product Information
FormatPurified
HS Code3002 15 90
Control
  • POSITIVE CONTROL: Conditioned, serum-free medium from (PMA-treated) human fibrosarcoma HT-1080 cells. Bladder, breast, ovarian carcinomas.
PresentationLiquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-MMP-13 Antibody, clone VIIIA2 is validated for use in IH for the detection of MMP-13.
Key Applications
  • Immunohistochemistry
Application NotesImmunohistochemistry (frozen and formalin-fixed/paraffin*): 2-4 μg/mL for 60 minutes at room temperature.

* Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Immunoprecipation: use protein G (2 μg/mg protein lysate)

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenHuman recombinant collagenase-3 protein.
CloneVIIIA2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityIt recognizes proteins of ~60 kDa and ~48 kDa which are identified as pro (latent) and activate forms of matrix metalloproteinase-13 (MMP-13; also known as Collagenase-3). Shows no cross reactivity with the pro and active forms of other MMPs. Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. The MMP-13 gene has been isolated and characterized. This gene is composed of 10 exons and 9 introns and spans over 12.5 kb. The overall organization of the collagenase-3 gene is similar to that of other MMP genes clustered at chromosome 11q22, including fibroblast collagenase (MMP-1), matrilysin (MMP-7), and macrophage metalloelastase (MMP-12), but is more distantly related to genes coding for stromelysin-3 (MMP-11), gelatinase-A (MMP-2), and gelatinase-B (MMP-9), which map outside of this gene cluster. Cellular Localization: Cytoplasmic
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
  • Rabbit
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
Gene Symbol
  • MMP13
  • MMP-13
  • CLG3
  • EC 3.4.24.-
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P45452 # Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
SIZE: 471 amino acids; 53820 Da
TISSUE SPECIFICITY: Seems to be specific to breast carcinomas.
DOMAIN: SwissProt: P45452 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
DISEASE: SwissProt: P45452 # Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia type 2 (SEMD2) [MIM:602111]; also known as spondyloepimetaphyseal dysplasia type Missouri. SEMDs are a heterogeneous group of skeletal disorders characterized by defective growth and modeling of the spine and long bones. The SEMDs are distinguished from the spondylometaphyseal dysplasias and the spondyloepiphyseal dysplasias by the combined involvement of the epiphyses and metaphyses. The 3 disorders have malformations of the vertebrae in common.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size100 µg
Global Trade Item Number
产品目录编号 GTIN
MAB13424 08436037126345