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ABE1851-25UG Anti-MLL-3

Anti-MLL3, Cat. No. ABE1851, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase 2C and has been tested in Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin), and Western Blotting.

Anti-MLL-3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Kmt2C, Mll3 UniProt Number: Q8BRH4
ABE1851-25UG
25 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RChIP, IH(P), WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE1851-25UG
DescriptionAnti-MLL-3
Alternate Names
  • Histone-lysine N-methyltransferase 2C
  • EC: 2.1.1.43
  • Lysine-N-methyltransferase 2C
  • Myeloid-lineage leukemia protein 3 homolog
Background InformationHistone-lysine N-methyltransferase 2C (UniProt: Q8BRH4; also known as EC: 2.1.1.43, Lysine-N-methyltransferase 2C, Myeloid-lineage leukemia protein 3 homolog, MLL-3) is encoded by the Kmt2c (also known as Mll3) gene in murine species. Mll3 gene is a member of the myeloid/lymphoid or mixed-lineage leukemia family. MLL-3 protein is a DHHC-type zinc finger protein with six PHD-type zinc fingers, a SET domain, a post-SET domain and a RING-type zinc finger. It methylates Lys-4 of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. MLL-3 is a central component of the MLL2/3 complex, a coactivator complex of nuclear receptors, involved in transcriptional co-activation. In adults, MLL-3 is detected in testis, kidney, spleen and lung and is weakly expressed in brain. It is absent in heart and liver tissue. MLL-3 has been reported to play a role in adipogenesis.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MLL3, Cat. No. ABE1851, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase 2C and has been tested in Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MLL-3 in mouse testis, rat uterus, and mouse kidney tissues.

Chromatin Immunoprecipitation Analysis: A representative lot detected MLL-3 in MEF cells (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8).

Western Blotting Analysis: A representative lot detected MLL-3 in Western Blotting applications (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8); Bres, V., et. al. (2009). Mol Cell. 36(1):75-87).

Immunohistochemistry Analysis: A representative lot detected MLL-3 in mouse urothelial tumors (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8).
Biological Information
ImmunogenA linear peptide corresponding to 18 amino acids from the N-terminal region of human MLL-3.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antiody detects MLL-3 protein in human, mouse and rat cells. It targets and epitope within 18 amino acids from the internal region of N-terminal half.
IsotypeIgG
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypePolyclonal Antibody
Gene Symbol
  • Kmt2C
  • Mll3
Purification MethodAffinity Purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in U2OS nuclear extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected MLL-3 in 10 µg of U2OS cell nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABE1851-25UG 04054839438028