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MABC1636-25UL Anti-MLKL Antibody, clone 7G2

Anti-MLKL, clone 7G2, Cat. No. MABC1636, is a rat monoclonal antibody that detects MLKL and is tested for use in Immunofluorescence and Western Blotting.

MABC1636-25UL
25 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABC1636-25UL
DescriptionAnti-MLKL Antibody, clone 7G2
Alternate Names
  • Mixed lineage kinase domain-like protein
Background InformationMixed lineage kinase domain-like protein (UniProt: Q8NB16; also known as hMLKL) is encoded by the MLKL gene (Gene ID: 197259) in human. MLKL is a pseudokinase that is a terminal protein in the pro-inflammatory necroptotic cell death program. It plays a critical role in TNFa-induced necroptosis. Although it contains a protein kinase domain (aa 194-469), it lacks several residues that are essential for protein kinase activity. It is activated following phosphorylation at Thr357/Ser358 by RIPK3, which leads to its homotrimerization and localization to the plasma membrane where it binds to highly phosphorylated inositol (InsP6) and cardiolipin and disrupts membrane integrity to cause necrotic cell death. Under basal conditions, MLKL is shown to reside in small puncta that are distributed evenly throughout the cytoplasm, but it is detected in plasma membrane shortly after its phosphorylation. It contains two coiled coil regions (aa 55-84 and 139-180). The second coiled coil region is responsible for its oligomerization. MLKL is also reported to be involved in endosomal trafficking, independently of induction of necroptosis, whereby it enhances degradation of receptors and ligands. Two isoforms of MLKL have been described that are produced by alternative splicing. (Ref.: Samson, AL., et al. (2020). Nat. Commun. 11(1); 3151; Yoon, S., et al. (2017). Immunity. 47(1); 51-65; Wang, H., et al (2014). Mol. Cell 54(1); 133-146).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-MLKL, clone 7G2, Cat. No. MABC1636, is a rat monoclonal antibody that detects MLKL and is tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected MLKL in Western Blotting applications (Samson, A.L., et al. (2020). Nat Commun. 11(1):3151).

Immunofluorescence Analysis: A representative lot detected MLKL in Immunofluorescence applications (Samson, A.L., et al. (2020). Nat Commun. 11(1):3151).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 282 amino acids from human Mixed lineage kinase domain-like protein (MLKL) expressed in baculovirus expression system.
EpitopeC-terminal
Clone7G2
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 7G2 is a rat monoclonal antibody that detects Human Mixed lineage kinase domain-like protein (MLKL). It targets an epitope within the C-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MLKL
Purification MethodProtein G purified
UniProt Number
Molecular Weight~50 kDa observed; 54.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysates from HT-29 cells.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected MLKL in lysates from wild-type HT-29 cells, but not in MLKL knockout cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABC1636-25UL 04065268041987