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ABN1018 Anti-LRP1 Antibody, Intracellular domain

Anti-LRP1 Antibody, Intracellular domain is an antibody against LRP1 for use in Western Blotting.

Anti-LRP1 Antibody, Intracellular domain MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: LRP1, A2MR, APR UniProt Number: Q07954
ABN1018
100 µL  
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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBRbSerumPolyclonal Antibody
Description
Catalogue NumberABN1018
DescriptionAnti-LRP1 Antibody, Intracellular domain
Alternate Names
  • Prolow-density lipoprotein receptor-related protein 1
  • A2MR
  • Alpha-2-macroglobulin receptor
  • APOER
  • Apolipoprotein E receptor
  • CD91
  • LRP-1
  • LRP-85
  • LRPICD
Background InformationProlow-density lipoprotein receptor-related protein 1 (UniProt Q07954; also known as A2MR, Alpha-2-macroglobulin receptor, APOER, Apolipoprotein E receptor, CD91, LRP-1) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID 4035) in human. LRP1 is an LDLR family member expressed by vascular endothelial cells forming the blood-brain barrier (BBB), vascular mural cells (pericytes and vascular smooth muscle cells (VSMCs)), neurons, and astrocytes. LRP1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (apoE), APP and Aβ. LRP1 is the primary receptor mediating transport of Aβ across the BBB into circulation, thereby clearing it from the brain. LRP1 is synthesized with a signal peptide sequence (a.a. 1-19), posttranslational cleavage yields a soluble form (sLRP1) and a cell surface-bound form composed of an 515 kDa extracellular α-chain (LRP-515) and a 85 kDa single-transmembrane (a.a. 4420-4444) β-chain (LRP-85) that contains the 100-amino intracellular domain (LRPICD; a.a. 4445-4544). BACE-1, ADAM-10, ADAM-12, ADAM-17, MT1-MMP, and t-PA are shown to be involved in the shedding of LRP1 to produce sLRP1.
References
Product Information
FormatSerum
PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-LRP1 Antibody, Intracellular domain is an antibody against LRP1 for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected the LRP-85 target band in NIH/3T3 cell lysate, as well as in mouse brain and liver homogenates (Courtesy of Professor Nicola Zambrano, University of Naples Federico II, Italy).
Western Blotting Analysis: A representative lot detected a decreased LRP-85 target band in lysates from LRP1 shRNA-treated NIH/3T3 cells (Courtesy of Professor Nicola Zambrano, University of Naples Federico II, Italy).
Biological Information
ImmunogenGST-tagged recombinant human LRP1 intracellular domain (LRPICD).
EpitopeLRPICD.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityRecognizes the Pro- and mature LRP-1 (a.a. 1-4544 and 20-4544, respectively), the beta-secretase-cleaved, membrane-bound LRP-85, and the gamma-secretase-cleaved intracellular domain LRPICD fragment, but not the beta-secretase-cleaved extracellular LRP-515 fragment.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human, Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • LRP1
  • A2MR
  • APR
Purification MethodUnpurified
UniProt Number
Molecular Weight~80 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected the LRP-85 target band in 10 µg of NIH/3T3 cell lysate.ysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABN1018 04055977349030