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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

LP02 Sigma-AldrichAnti-LDL Receptor Mouse mAb (15C8)

This Anti-LDL Receptor Mouse mAb (15C8) is validated for use in ELISA, Flow Cytometry, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of LDL Receptor.

Anti-LDL Receptor Mouse mAb (15C8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书
参考
Data Sheet

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
B, H	M	Monoclonal Antibody

Products

产品目录编号	包装	数量 / 包装
LP02-100UGCN	塑胶安瓿;塑胶针药瓶	100 μg	添加到收藏夹添加到收藏夹添加到我的收藏夹

Description
Overview	Recognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells.
Catalogue Number	LP02
Brand Family	Calbiochem®
Application Data	Detection of human LDL receptor by immunocytochemistry. Sample: HepG2 cells fixed in methanol. Primary antibody: Anti-LDL Receptor Mouse mAb (15C8) (Cat. No. LP02) (5 µg/ml). Detection: fluorescence.

References
References	van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127. van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443. Russell, D.W., et al. 1984. Cell 37, 577. Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635. Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261. Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664. Goldstein, J.L., et al. 1979. Nature 279, 679.

References

van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127.
van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443.
Russell, D.W., et al. 1984. Cell 37, 577.
Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635.
Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261.
Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664.
Goldstein, J.L., et al. 1979. Nature 279, 679.

Product Information
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive control	Liver sections, HT1080 cells, HepG2 cells
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Original Clone Russell, D.W., et al. 1984. Cell 37, 577. Specificity van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127. Immunoblotting van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443. Flow Cytometry Duvillard, L., et al. 2003. Diabetes Care 26, 1540.
Key Applications	Enzyme-Linked Immunosorbent Assay Flow Cytometry Frozen Sections Immunoblotting (Western Blotting) Immunocytochemistry Immunoprecipitation
Application Notes	ELISA (0.5-1 µg/ml) Flow Cytometry (see application references) Frozen Sections (1-5 µg/ml) Immunoblotting (1-5 µg/ml, see application references) Immunocytochemistry (1-5 µg/ml, IF) Immunoprecipitation (1-2 µg/sample)
Application Comments	Will displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca⁺² with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a trypic digest of bovine low density lipoprotein receptor
Immunogen	Bovine
Epitope	first cysteine-rich ligand binding domain
Clone	15C8
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Bovine Human
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
LP02-100UGCN	04055977228076

Documentation

Anti-LDL Receptor Mouse mAb (15C8) 分析证书

标题	批号
LP02	输入批号

参考

参考信息概述
van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127. van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443. Russell, D.W., et al. 1984. Cell 37, 577. Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635. Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261. Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664. Goldstein, J.L., et al. 1979. Nature 279, 679.

参考信息概述

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	12-June-2008 JSW
Application	ELISA (0.5-1 µg/ml) Flow Cytometry (see application references) Frozen Sections (1-5 µg/ml) Immunoblotting (1-5 µg/ml, see application references) Immunocytochemistry (1-5 µg/ml, IF) Immunoprecipitation (1-2 µg/sample)
Application Data	Detection of human LDL receptor by immunocytochemistry. Sample: HepG2 cells fixed in methanol. Primary antibody: Anti-LDL Receptor Mouse mAb (15C8) (Cat. No. LP02) (5 µg/ml). Detection: fluorescence.
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein.
Background	The low density lipoprotein receptor is responsible for mediating the uptake of LDL, a cholesterol carrying plasma lipoprotein. The receptor is a member of a class of cell surface proteins which are concentrated in clathrin coated pits and carry external ligands into cells by receptor-mediated endocytosis. The purified receptor is a glycoprotein with a molecular weight of 160,000. It is synthesized as a precursor of molecular weight 120,000 that is then glycosylated to the mature form by addition of N-linked and O-linked sugars in the Golgi apparatus. Unlike other hepatic lectins, the orientation of the LDL-R is such that the amino terminus is to the exterior of the cell. It has recently been determined that the LDL-R has sequence homology with the epidermal growth factor receptor precursor.
Host	Mouse
Immunogen species	Bovine
Immunogen	a trypic digest of bovine low density lipoprotein receptor
Epitope	first cysteine-rich ligand binding domain
Clone	15C8
Isotype	IgG_2a
Species	bovine, human, not rabbit
Positive control	Liver sections, HT1080 cells, HepG2 cells
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Will displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca⁺² with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127. van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443. Russell, D.W., et al. 1984. Cell 37, 577. Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635. Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261. Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664. Goldstein, J.L., et al. 1979. Nature 279, 679.
Application references	Original Clone Russell, D.W., et al. 1984. Cell 37, 577. Specificity van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127. Immunoblotting van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443. Flow Cytometry Duvillard, L., et al. 2003. Diabetes Care 26, 1540.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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