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MABN2432-25UG Anti-Kv3.2 Antibody, clone N410/17

Anti-Kv3.2, clone N410/17, Cat. No. MABN2432, is a highly specific mouse monoclonal antibody that targets Voltage-gated potassium channel subunit Kv3.2 and has been tested in Western Blotting and Immunohistochemistry (Paraffin).

MABN2432-25UG
25 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABN2432-25UG
DescriptionAnti-Kv3.2 Antibody, clone N410/17
Alternate Names
  • Potassium voltage-gated channel subfamily C member 2
  • Potassium channel voltage-gated Shaw-related subfamily C member 2
  • Shaw-like potassium channel
  • Voltage-gated potassium channel subunit Kv3.2.
Background InformationPotassium voltage-gated channel subfamily C member 2 (UniProt: P22462; also known as Potassium channel voltage-gated Shaw-related subfamily C member 2, Shaw-like potassium channel, Voltage-gated potassium channel subunit Kv3.2) is encoded by the Kcnc2 gene (Gene ID: 246153) in rat. Potassium voltage-gated channel 3.2 is a multi-pass membrane protein that is widely expressed in the brain. It mediates transmembrane potassium transport in excitable membranes and contributes to the regulation of the fast action potential repolarization and in sustained high-frequency firing in neurons of the central nervous system. It is usually present as a homotetramer, but can also form heterotetramer with other channel-forming alpha subunits, such as Kv3.1. Homotetrameric channels mediate delayed-rectifier voltage-dependent potassium currents that activate rapidly at high-threshold voltages and inactivate slowly. The channel is reported to alternate between opened and closed conformations in response to the voltage difference across the membrane. The channel is reported to alternate between opened and closed conformations in response to the voltage difference across the membrane. Channel opening and closing is affected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by transmembrane domains S3 and S4. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Kv3.2 is phosphorylated by protein kinase A, which leads to inhibition of channel activity.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Kv3.2, clone N410/17, Cat. No. MABN2432, is a highly specific mouse monoclonal antibody that targets Voltage-gated potassium channel subunit Kv3.2 and has been tested in Western Blotting and Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Kv3.2 in rat brain tissue sections.
Biological Information
ImmunogenA recombinant fragment corresponding to 140 amino acids from the C-terminal region of rat Voltage-gated potassium channel subunit Kv3.2.
CloneN410/17
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone N410/17 is a mouse monoclonal antibody that specifically detects Voltage-gated potassium channel subunit Kv3.2. It targets an epitope with in the C-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Rat
  • Human
Species Reactivity NoteHuman, Mouse, Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Kcnc2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~67 kDa observed; 67.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Kv3.2 in rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABN2432-25UG 04054839986734

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