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MABN2729-25UG Sigma-AldrichAnti-Islet-1 homeobox Antibody, clone 40.2D6

Anti-Islet-1 homeobox, clone 40.2D6, Cat. No. MABN2729, is a mouse monoclonal antibody that detects Islet-1 homeobox and is tested in ChIP, EMSA, Flow Cytometry, Immunofluorescence, Immunohistochemist

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概述

Description
Catalogue Number	MABN2729-25UG
Description	Anti-Islet-1 homeobox Antibody, clone 40.2D6
Alternate Names	Insulin gene enhancer protein ISL-1
Background Information	Insulin gene enhancer protein ISL-1 (UniProt: P61374; also known as Islet-1) is encoded by the Isl1 gene (Gene ID: 64444) in rat. Islet-1 is a DNA-binding transcriptional activator that is expressed in pancreatic islets and is essential for the maturation, proliferation, and survival of the endocrine pancreas. It recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Its DNA binding region is localized in amino acids 181-240. It also contains two LIM Zinc-binding domains (aa 17-79 and 90-133) and a LIM-binding domain (aa 262-291). It plays a key role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. It cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. It is also involved in the specification of motor neurons in cooperation with LHX3 and LDB1. It is reported to be essential for cardiac development and is considered as a marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells. It also controls the expression of fibroblast growth factor and bone morphogenetic protein. Islet-1 expression is a reliable marker for pancreatic endocrine tumors and their metastases. Two isoforms of islet-1 have been described that are produced by alternative splicing. (Ref.: Schmitt, AM., et al. (2008). Am. J. Surg. Pathol. 32(3); 420-425; Karlsson, O., et al. (1990). Nature. 344(6269); 879-882).

Product Information
Format	Purified
Presentation	Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ200

Applications
Application	Anti-Islet-1 homeobox, clone 40.2D6, Cat. No. MABN2729, is a mouse monoclonal antibody that detects Islet-1 homeobox and is tested in ChIP, EMSA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry, and Western Blotting.
Key Applications	ChIP-seq Electrophoretic Mobility Shift Assay Flow Cytometry Immunofluorescence Immunohistochemistry (Paraffin) Immunoprecipitation Immunocytochemistry Western Blotting
Application Notes	Tested Applications Immunohistochemistry Applications: A representative lot detected Islet-1 homeobox in Immunohistochemistry applications (Hoeber, J., et al. (2015). Sci Rep. 5:10666; Fischer, A.J., et al. (2010). PLoS One. 5(6):e10774; Schick, E., et al. (2019). Sci Rep. 9(1):9358; Ediger, B.N., et al. (2014). Diabetes. 63(12):4206-17; Wan, Y., et al. (2019). Cell. 179(2):355-372.e23). Immunocytochemistry Analysis: A representative lot detected Islet-1 homeobox in Immunocytochemistry applications (Vahsen, B.F., et al. (2022). Sci Rep. 12(1):12606; Hoeber, J., et al. (2015). Sci Rep. 5:10666). Immunohistochemistry (Paraffin) Analysis: A representative lot detected Islet-1 homeobox in Immunohistochemistry applications (Du, A., et al. (2009). Diabetes. 58(9):2059-69; Ediger, B.N., et al. (2014). Diabetes. 63(12):4206-17). Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected Islet-1 homeobox in Chromatin Immunoprecipitation applications (Du, A., et al. (2009). Diabetes.58(9):2059-69). Immunoprecipitation Analysis: A representative lot immunoprecipitated Islet-1 homeobox in Immunoprecipitation applications (Velasco, S., et al. (2017). Cell Stem Cell. 20(2):205-217). Western Blotting Analysis: A representative lot detected Islet-1 homeobox in Western Blotting applications (Haremaki, T., et al. (2010). Dev Dyn. 239(7):1977-87). Electrophoretic Mobility Shift Assay (EMSA): A representative lot detected Islet-1 homeobox in Electrophoretic Mobility Shift Assay applications (Ediger, B.N., et al. (2014). Diabetes. 63(12):4206-17). Flow Cytometry Analysis: A representative lot detected Islet-1 homeobox in Flow Cytometry applications (Meng, X., et al. (2020). Genes (Basel). 11(3):328). Immunofluorescence Analysis: A representative lot detected Islet-1 homeobox in Immunofluorescence applications (Vahsen, B.F., et al. (2022). Sci Rep. 12(1):12606; Hoeber, J., et al. (2015). Sci Rep. 5:10666; Du, A., et al. (2009). Diabetes. 58(9):2059-69; Ediger, B.N., et al. (2014). Diabetes. 63(12):4206-17). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biological Information
Immunogen	A recombinant fragment corresponding to 172 amino acids from the C-terminal half of rat Insulin gene enhancer protein ISL-1.
Epitope	C-terminal half
Clone	40.2D6
Concentration	0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host	Mouse
Specificity	Clone 40.2D6 is a mouse monoclonal antibody that detects Insulin gene enhancer protein ISL-1. It targets an epitope within the C-terminal half.
Isotype	IgG1κ
Species Reactivity	Human Frog Canine Guinea Pig Mouse Rhesus Macaque Rat Zebrafish
Species Reactivity Note	Human, Frog, Canine, Guinea Pig, Mouse, Rhesus Macaque, Rat, Zebra Fish.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_059035
Gene Symbol	Isl1
Purification Method	Protein G purified
UniProt Number	P61374
Molecular Weight	39.04 kDa calculated.

Product Usage Statements
Quality Assurance	Evaluated by Immunohistochemistry (Paraffin) in Human pancreas tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Islet-1 homeobox protein in Human pancreas tissue sections.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage: +2°C to +8°C.

Packaging Information
Material Size	25 μg

Global Trade Item Number
产品目录编号	GTIN
MABN2729-25UG	04065270845399

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