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MAC139-25UG Anti-Inntag™ 6 (IT6) Antibody, clone R19/4-11/15

Anti-Inntag™ 6 (IT6), clone R19/4-11/15 Antibody, Cat. No. MAC139-25UG, validated for use in Western Blotting, Immunocytochemistry and Immunoprecipitation.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: HEV1 UniProt Number: P02877
MAC139-25UG
25 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, ICC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAC139-25UG
DescriptionAnti-Inntag™ 6 (IT6) Antibody, clone R19/4-11/15
Alternate Names
  • Major hevein
Background InformationPro-hevein (UniProt: P02877; also known as Major hevein) is encoded by the HEV1 gene in Hevea brasiliensis (Para rubber tree). Pro-hevein is a N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin that can inhibit fungal growth. It is known to cause allergic reaction in human. It contains one barwin domain and one chitin-binding type-1 domain. Inntag ™ 6 IT6 is a small protein domain of Hevea brasiliensis that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it is not shown to compromise cell fitness when overexpressed and has no detectable functional and localization effects when fused to a variety of target proteins. Unlike the monomeric HA, Flag and MYC tags, Inntag ™ IT6 does not increase the intrinsic variability of critical size in budding yeast cells. Structure of the Inntag ™ IT6 is based on a packed beta-sheet conformation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
Quality LevelMQ100
Applications
ApplicationAnti-Inntag™ 6 (IT6), clone R19/4-11/15 Antibody, Cat. No. MAC139-25UG, validated for use in Western Blotting, Immunocytochemistry and Immunoprecipitation.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: A representative lot detected Inntag ™ 6 (IT6) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8). Immunoprecipitation Analysis: A representative lot detected Inntag ™ 6 (IT6) in Immunoprecipitation applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8). Immunofluorescence Analysis: A representative lot detected Inntag ™ 6 (IT6) in Immunofluorescence applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
Biological Information
ImmunogenHis-tagged recombinant peptide fragment corresponding to 43 amino acids from Hevea brasiliensis.
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone R19/4-11/15 detecs Pro-hevein in HEK293 cells tranfected with GFP-IT6.
IsotypeIgG2b
Species Reactivity
  • All
Species Reactivity NoteHevea brasiliensis.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • HEV1
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in transfected lysates from HEK293cells transfected with GFP-IT6.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MAC139-25UG 04054839420931