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MABF3175-100UG Anti-IL-5 Antibody, clone 4A1.6.15

MABF3175-100UG
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABF3175-100UG
DescriptionAnti-IL-5 Antibody, clone 4A1.6.15
Alternate Names
  • Interleukin-5
  • B-cell differentiation factor I
  • Eosinophil differentiation factor
  • T-cell replacing factor
  • TRF
Background InformationInterleukin-5 (UniProt: P05113; also known as IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF) is encoded by the IL5 gene (Gene ID: 3567) in human. IL-5 is a disulfide-linked homodimeric secreted protein that is synthesized and secreted mainly by the activated CD4+ helper T cells of the Th2 subset, mast cells, and eosinophils. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein (aa 20-134). It serves as a powerful proinflammatory cytokine that is responsible for maturation, proliferation, activation, and migration of eosinophils. It is also a key factor in the upregulation of myeloid progenitors in the bone marrow after airway allergen challenge, and for trafficking from the marrow to the airways in upper or lower airways inflammation. Its biological effects are mediated via selective interaction with IL-5 receptor (IL-5R), which is composed of an a subunit (IL-5Ra) and a non-specific βc heterodimer. As a homodimer it binds to IL-5Ra expressed on eosinophil surface and recruits the βc dimer and induces the assembly of the IL-5/IL-5Ra/βc ternary complex. In the absence of IL-5, IL-5Ra is complexed with the intracellular JAK2, whereas the βc subunit is associated with JAK1. (Ref.: Pelaia, C., et al. (2019). Front. Physiol. 10; 1514).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-IL-5, clone 4A1.6.15, Cat. No. MABF3175, is a mouse monoclonal antibody that detects human Interleukin-5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesTested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-5 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length recombinant human Interleukin-5 (without the signal peptide).
EpitopeUnknown
Clone4A1.6.15
Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 4A1.6.15 is a mouse monoclonal antibody that detects human Interleukin-5 (IL-5).
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • IL5
Purification MethodProtein G purified
UniProt Number
Molecular Weight~26 kDa observed; 15.24 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant human IL-5. Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human IL-5.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABF3175-100UG 04065267463759