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393207 Anti-HNE-Michael Adducts, Reduced Rabbit pAb

Anti-HNE-Michael Adducts, Reduced, rabbit polyclonal, specifically recognizes chemically reduced amino acid-(4)-HNE adducts. It is validated for use in ELISA, WB & IHC (frozen & paraffin secti

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Anti-HNE-Michael Adducts, Reduced Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: Anti-4-Hydroxy-2-noneal Michael Adducts, Reduced

393207
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概述

重要规格表

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesRbPolyclonal Antibody

Products

产品目录编号包装 数量 / 包装
393207-100ULCN 塑胶安瓿;塑胶针药瓶 100 ul
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Description
OverviewRecognizes chemically reduced amino acid-(4)-HNE adducts. Does not recognize non-reduced adducts or reduced modifications arising from malondialdehyde (MDA) and structurally-related alkenes such as trans-2-noneal.
Catalogue Number393207
Brand Family Calbiochem®
SynonymsAnti-4-Hydroxy-2-noneal Michael Adducts, Reduced
References
ReferencesToyokuni, S., et al. 2000. Antioxid. Redox. Signal 2, 681.
Neely, M.D., et al. 1999. J. Neurochem. 72, 2323.
Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun. 212, 1068.
Uchida, K., et al. 1995. Arch. Biochem. Biophys. 324, 241.
Uchida, K., et al. 1994. Biochemistry 33, 12487.
Product Information
FormLiquid
FormulationSerum diluted in 50% glycerol, pH 7.5.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (1:500-1:1000)
Immunoblotting (1:1000-1:4000)
Frozen Sections (1:50-1:500)
Paraffin Sections (1:50-1:500)
Application CommentsDoes not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
ImmunogenHNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced
HostRabbit
IsotypeIgG
Species Reactivity
  • A Broad Range Of Species
Antibody TypePolyclonal Antibody
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
产品目录编号 GTIN
393207-100ULCN 04055977212495