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MABC1035 Anti-Gremlin-1 Antibody, clone 2H1.1

Anti-Gremlin-1, clone 2H1.1, Cat. No. MABC1035, is a mouse monoclonal antibody that detects Gremlin-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Gremlin-1 Antibody, clone 2H1.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: GREM1, CKTSF1B1, DAND2, DRM, PIG2 UniProt Number: O60565
MABC1035
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1035
DescriptionAnti-Gremlin-1 Antibody, clone 2H1.1
Alternate Names
  • Cell proliferation-inducing gene 2 protein
  • Cysteine knot superfamily 1
  • BMP antagonist 1
  • DAN domain family member 2
  • Down-regulated in Mos-transformed cells protein
  • Increased in high glucose protein 2
  • IHG-2
Background InformationGremlin-1 (UniProt: O60565; also known as Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, BMP antagonist 1, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, Increased in high glucose protein 2, IHG-2) is encoded by the GREM1 (also known as CKTSF1B1, DAND2, DRM, PIG2) gene (Gene ID: 26585 ) in human. Gremlin-1 is a homodimeric cytokine that plays an important role during carcinogenesis and metanephric kidney organogenesis. Two isoforms of Gremlin-1 have been described that are produced by alternative splicing. Gremlin-1 acts as an antagonist of BMP2 and inhibits BMP2-mediated differentiation of osteoblasts. It also down-regulates the BMP4 signaling in a dose-dependent manner. Gremlin-1 is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off. Gremlin-1 is highly expressed in small intestine where its expression is restricted to intestinal subepithelial myofibroblasts (ISEMFs) at the crypt base. It is also expressed in fetal brain and colon and weak expression is observed in adult brain ovary, prostate, pancreas, and skeletal muscle. Its levels are down-regulated in tumor cell lines. Mutations in GREM1 gene are known to cause Polyposis syndrome, mixed hereditary 1 (HMPS1) that is characterized by apparent autosomal dominant inheritance of multiple types of colorectal polyp, with colorectal carcinoma occurring in a high proportion of affected individuals.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Gremlin-1, clone 2H1.1, Cat. No. MABC1035, is a mouse monoclonal antibody that detects Gremlin-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Gremlin-1 in human prostate and human colon tissues.
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 89 amino acids from the internal region.
Clone2H1.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 2H1.1 detects Gremlin-1 in human cells. It targets an epitope within 89 amino acids from the internal region of isoform 1.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GREM1
  • CKTSF1B1
  • DAND2
  • DRM
  • PIG2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~36 kDa observed; 20.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Gremlin recombinant protein.

Western Blotting Analysis: 1 µg/mL of this antibody detected Gremlin-1 recombinant protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABC1035 04054839415272