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MABN1189 Anti-GluR2 Antibody, clone 14C12.2

Anti-GluR2, clone 14C12.2, Cat. No. MABN1189, is a highly specific mouse monoclonal antibody, that targets Glutamate Receptor 2 and has been tested in Western Blotting and Immunohistochemistry (Paraffin).

Anti-GluR2 Antibody, clone 14C12.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Gria2, Glur2 UniProt Number: P19491
MABN1189
100 μg  
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概述

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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, H, MIH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1189
ReplacesMAB397
DescriptionAnti-GluR2 Antibody, clone 14C12.2
Alternate Names
  • Glutamate receptor 2
  • AMPA-selective glutamate receptor 2
  • GluA2
  • GluR-2
  • GluR-B
  • GluR-K2
  • GluR2
  • Glutamate receptor ionotropic, AMPA 2
Background InformationGlutamate receptor 2 (UniProt P19491; also known as AMPA-selective glutamate receptor 2, GluA2, GluR-2, GluR-B, GluR-K2, GluR2, Glutamate receptor ionotropic, AMPA 2) is encoded by the Gria2 (also known as Glur2) gene (Gene ID 29627) in rat species. Glutamate receptors are synaptic receptors located primarily on the membranes of neuronal cells. These receptors play important roles in neural communication, memory formation, learning, and regulation by mediating glutamate-dependent postsynaptic excitation. Glutamate receptors are divided into two types, ionotropic glutamate receptors (iGluRs) and metabotropic glutamate receptors (mGluRs). iGluRs form ion channel pores upon glutamate binding, while mGluRs indirectly activate ion channels on the plasma membrane via G protein-coupled signaling cascade. Although all iGluRs and mGluRs bind glutamate, they do exhibit different binding activities toward chemical ligands, such as NMDA, kainate, AMPA, L-AP4, ACPD, L-QA, and such distinciton is used to divide these receptors further into subtypes. Glutamate receptor 2 is an AMPA type iGluR, GluR-1 is initially produced with an N-terminal signal peptide sequence (a.a. 1-24), the removal of which yields the mature receptor with 4 transmembrane helices (a.a. 544-564, 592-610, 617-637, 813-833), having a large N-terminal extracellular domain (a.a. 24-543), followed by two intracellular loops (a.a. 565-591, 611-616), one extracellular loop (a.a. 638-812) and a C-terminal cytoplasmic tail (a.a. 834-883).
References
Product Information
FormatPurified
PresentationPurified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GluR2, clone 14C12.2, Cat. No. MABN1189, is a highly specific mouse monoclonal antibody, that targets Glutamate Receptor 2 and has been tested in Western Blotting and Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected GluR2 in mouse (hippocampus) and human (cerebellum and cerebral cortex) brain tissue sections.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected GluR2 in 10 µg of rat brain membrane extract.
Biological Information
ImmunogenGST-tagged recombinant rat GluR2 N-terminal extracellular domain fragment.
Clone14C12.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 14C12.2 targets an epitope within the N-terminal extracellular domain present in all spliced isoforms of human, mouse, and rat GluR2 reported by UniProt (P42262, P23819, P19491).
IsotypeIgG2aκ
Species Reactivity
  • Rat
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Gria2
  • Glur2
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~110 kDa observed. Target band size appears larger than the caluclated molecular weights (signal peptide removed) of 96.18/96.24/97.96/93.78 kDa (human isoform Flop/Flip/3/4), 96.02/83.54/101.9/100.2 kDa (mouse isoform 1/2/3/4), 90.05/96.11/97.78 kDa (rat isoform Flop/Flip/3) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain membrane extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected GluR2 in 10 µg of mouse brain membrane extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABN1189 04054839083617

Documentation

Anti-GluR2 Antibody, clone 14C12.2 MSDS

职位

物料安全数据表 (MSDS) 

Anti-GluR2 Antibody, clone 14C12.2 分析证书

标题批号
Anti-GluR2, clone 14C12.2 3150359
Anti-GluR2, clone 14C12.2 3083571
Anti-GluR2, clone 14C12.2 - 3172415 3172415
Anti-GluR2, clone 14C12.2 - 3256601 3256601
Anti-GluR2, clone 14C12.2 - 3429037 3429037
Anti-GluR2, clone 14C12.2 - 3469436 3469436
Anti-GluR2, clone 14C12.2 - 3508323 3508323
Anti-GluR2, clone 14C12.2 - 3697302 3697302
Anti-GluR2, clone 14C12.2 - 3923535 3923535
Anti-GluR2, clone 14C12.2 - 3977207 3977207
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