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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABC667 Sigma-AldrichAnti-GPC3 Antibody, clone 9C2

Detect Glypican using this mouse monoclonal antibody, Anti-GPC3 Antibody, clone 9C2 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.

Anti-GPC3 Antibody, clone 9C2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: GPC3, OCI5 UniProt Number: P51654

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, IF, IHC, FC	M	Ascites	Monoclonal Antibody

Description
Catalogue Number	MABC667
Description	Anti-GPC3 Antibody, clone 9C2
Alternate Names	SGB DGSX MXR7 SDYS SGBS OCI-5 SGBS1 GTR2-2 Glypican-3 Intestinal protein OCI-5
Background Information	Glypican-3 or GTR2-2 is the protein product of the gene GPC3. It is a cell surface heparin sulfate containing glycoprotein that plays an important role in the modulation of growth and development of mesodermal tissues and organs, as well as various cancers. Among its many known functions, it is a regulator of the dipeptidyl peptidase DPP4 (CD26). DPP4 plays an important role in glucose metabolism via its action on incretin and GLP-1 hormones. DPP4 also is a cancer marker and thought to function as a cancer suppressor. Thus acting as a regulator of DPP4, Glypican-3 (GPC3) participates in everything from glucose and blood sugar regulation to tumor growth. Glypican-3 (GPC3) is most highly expressed in the lung, liver and kidney, and typically a secreted form, secreted glypican-3, is found in the extracellular space generated as a cleavage product by the action of a convertase. Defects in GPC3 cause Simpson-Golabi-Behmel syndrome type 1 (SGBS1) or Simpson dysmorphia syndrome (SDYS). This is a condition characterized by pre-and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. EMD-Millipore’s anti-GPC3 mouse monoclonal antibody, clone 9C2, has been tested in western blot against recombinant human protein, human HepG2, HEK293, Jurkat, and SK-N-SH cell line lysates, as well as rat PC-12 and mouse liver cell lysates. In addition, the clone as been successfully used in paraffin-embedded immunohistochemistry on human liver and breast cancer tissues and in immunofluorescent immunocytochemistry on human HeLa cells in culture. Finally, the clone has been tested successfully on human cells by flow cytometry and against the recombinant antigen by ELISA.

References

Product Information
Format	Ascites
Control	Mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates
Presentation	Purified mouse monoclonal in PBS with 0.05% sodium azide
Quality Level	MQ100

Applications
Application	Detect Glypican using this mouse monoclonal antibody, Anti-GPC3 Antibody, clone 9C2 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Key Applications	Western Blotting Immunofluorescence Immunohistochemistry Flow Cytometry
Application Notes	Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected GPC3 in human liver and kidney tissues. Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GPC3 in HeLa cells. Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected GPC3 in Jurkat cells. Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Purified recombinant fragment of human GPC3 expressed in E. Coli.
Clone	9C2
Host	Mouse
Isotype	IgG1
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody
Gene Symbol	GPC3 OCI5
Purification Method	Protein G Purified
UniProt Number	P51654
Molecular Weight	~66 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates. Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPC3 in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABC667	04053252918056

Documentation

Anti-GPC3 Antibody, clone 9C2 MSDS

职位
物料安全数据表 (MSDS)

Anti-GPC3 Antibody, clone 9C2 分析证书

标题	批号
Anti-GPC3, clone 9C2 - VP1401035	VP1401035
Anti-GPC3, clone 9C2 - VP1404281	VP1404281
Anti-GPC3, clone 9C2 - QVP1305307	QVP1305307
Anti-GPC3, clone 9C2 - VP1905161	VP1905161
Anti-GPC3, clone 9C2 - VP1909200	VP1909200
Anti-GPC3, clone 9C2 - VP2112011	VP2112011
Anti-GPC3, clone 9C2 - VP2202171	VP2202171
Anti-GPC3, clone 9C2 - VP2205192	VP2205192
Anti-GPC3, clone 9C2 - VP2210043	VP2210043
Anti-GPC3, clone 9C2 - VP2303020	VP2303020

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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