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MAB1926-I Anti-Fibronectin Antibody, cell binding domain Antibody, clone P1H11

Anti-Fibronectin, cell binding domain, clone P1H11, Cat. No. MAB1926-I is a mouse monoclonal antibody that detects human fibronectin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

MAB1926-I
200 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMAB1926-I
DescriptionAnti-Fibronectin Antibody, cell binding domain Antibody, clone P1H11
Alternate Names
  • FN
  • Cold-insoluble globulin
  • CIG
Background InformationFibronectin (UniProt: P02751; also known as FN, Cold-insoluble globulin, CIG) is encoded by the FN1 (also known as FN) gene (Gene ID: 2335) in human. Fibronectin is are involved various biological activities, including cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. It is essential for osteoblast mineralization and participates in the regulation of type I collagen deposition by osteoblasts. Fibronectin is usually present as homodimer of ~250 kDa subunits where subunits are connected by two disulfide bonds at the C-terminal region. Each monomer contains three repeating units, which are designated as type I, II, and III repeats containing about 40, 60, and 90 amino acids, respectively. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes and the cellular fibronectin (dimeric or cross-linked multimeric forms) is produced by fibroblasts, epithelial and other cell types. Cellular fibronectin is deposited as fibrils in the extracellular matrix. Fibronectin is recognized by integrins alpha5beta1 and alphaVbeta3. The primary sequence motif of fibronectin for integrin binding is a tripeptide, Arg-Gly-Asp (RGD). Mutations in FN gene are known to cause glomerulopathy that is characterized by proteinuria, microscopic hematuria, and hypertension, which can lead to end-stage renal failure.
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Fibronectin, cell binding domain, clone P1H11, Cat. No. MAB1926-I is a mouse monoclonal antibody that detects human fibronectin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Fibronectin in human kidney and human cerebellum tissues.

Western Blotting Analysis: A representative lot detected Fibronectin in Western Blotting applications (Zhang, H., et. al. (2010). Am J Physiol Renal Physiol. 299(1):F91-8).

Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Mardilovich, A., et. al. (2006). Langmuir. 22(7):3259-64).

Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Hofanson, D.M., et. al. (2010). Biomaterials. 31(26):6730-7).

Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Waterman, R.S., et. al. (2010). PLoS One. 5(4):e10088).

Immunohistochemistry Analysis: A representative lot detected Fibronectin in Immunohistochemistry applications (Carlos, C.P., et. al. (2014). PLoS One. 9(7):e103660).

Western Blotting Analysis: A representative lot detected Fibronectin in Western Blotting applications (Zhang, H., et. al. (2008). Am J Physiol Renal Physiol. 295(4):F1071-81).
Biological Information
ImmunogenHuman Plasma Fibronectin.
CloneP1H11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone P1H11 detects fibronectin in human.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
  • Porcine
Species Reactivity NoteHuman, Mouse, Porcine.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FN1
  • FN
Purification MethodProtein G purified
UniProt Number
Molecular Weight~270 kDa observed; 262.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human thymus tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Fibronectin in 10 µg of human thymus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MAB1926-I 04054839337161

Documentation

Anti-Fibronectin Antibody, cell binding domain Antibody, clone P1H11 MSDS

职位

物料安全数据表 (MSDS) 

Anti-Fibronectin Antibody, cell binding domain Antibody, clone P1H11 分析证书

标题批号
Anti-Fibronectin, cell binding domain, clone P1H11 - 3379916 3379916
Anti-Fibronectin, cell binding domain, clone P1H11 - 3719553 3719553
Anti-Fibronectin, cell binding domain, clone P1H11 - 3849497 3849497
Anti-Fibronectin, cell binding domain, clone P1H11 Monoclonal Antibody 3107363

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