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05-338 Anti-Fas Antibody (human, neutralizing), clone ZB4

05-338
100 µg  
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FC, NEUTMPurifiedMonoclonal Antibody
Description
Catalogue Number05-338
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Fas Antibody (human, neutralizing), clone ZB4
OverviewThis product does not induce apoptosis in cell culture.
Alternate Names
  • APO-1 cell surface antigen
  • Apo-1 antigen
  • Apoptosis-mediating surface antigen FAS
  • CD95 antigen
  • FASLG receptor
  • Fas (TNF receptor superfamily, member 6)
  • Fas AMA
  • Fas antigen
  • apoptosis antigen 1
  • tumor necrosis factor receptor superfamily member 6
  • tumor necrosis factor receptor superfamily, member 6
Background InformationFas (also known as Apo-1 and CD95) is a 40-50 kDa cell surface glycoprotein and a member of the TNF receptor family. Fas is an apotosis signalling molecule that forms the death inducing signalling complex (DISC) upon binding by the Fas ligand trimer on an adjacent cell. The Fas receptor also trimerizes, after which it is internalized and can then bind to the adaptor molecule FADD. FADD facilitates binding of caspase-8. which is activated to induce caspase-8 mediated apoptosis. Fas also plays a role in T cell mediated cytotoxicity and T cell development. Fas is found in the thymus, liver, heart, and ovary, where it is expressed on a minority of resting T and B cells and monocytes. The antagonistic ZB4 clone anti-Fas monoclonal has been used to block the apoptosis-inducing activity of the Anti-Fas CH-11 clone in multiple publications.
References
Product Information
FormatPurified
Control
  • Various human cells
PresentationAmmonium sulfate and purified mouse monoclonal IgG1 in PBS containing 50% glycerol. Store at -20°C.
Quality LevelMQ100
Applications
ApplicationAnti-Fas Antibody (human, neutralizing), clone ZB4 detects level of Fas & has been published & validated for use in WB, FC, NEUT.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • Neutralizing
Application NotesFlow Cytometry: A previous lot of this antibody used 5-20 μg/mL. Western Blot: A previous lot of this antibody used 5 μg/mL.
Biological Information
ImmunogenRecombinant human Fas.
Cloneclone ZB4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lympho-blastoid cells and diploid fibroblasts. It does not induce apoptosis in cell culture.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman. This antibody does not recognize TNF and mouse Fas.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
Gene Symbol
  • FAS
  • OTTHUMP00000059646
  • LFG
  • FASTM
  • ALPS1A
  • TNFRSF6
  • CD95
  • FAS1
  • FAIM2
  • APT1
  • NMP35
  • APO-1
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P25445 # Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
SIZE: 335 amino acids; 37732 Da
SUBUNIT: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BRE.
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted. & Isoform 6: Secreted.
TISSUE SPECIFICITY: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
DOMAIN: SwissProt: P25445 Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
DISEASE: SwissProt: P25445 # Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
SIMILARITY: Contains 1 death domain. & Contains 3 TNFR-Cys repeats.
Molecular Weight45 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Availability by Geography
  • This product is not available for sale in Japan.
Quality AssuranceEvaluated by neutralization of apoptosis induced by anti-Fas (human, activating), clone CH11 (Catalog # 05-201) treatment of human Jurkat cells.

Neutralization: 10-500 ng/mL of this antibody was incubated for one hour with human Fas-transfected cells and inhibited the anti-Fas (human, activating), clone CH11 (Catalog # 05-201). Cell viability was assessed using a WST-1 assay
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
05-338 04053252336454

Documentation

Anti-Fas Antibody (human, neutralizing), clone ZB4 MSDS

职位

物料安全数据表 (MSDS) 

Anti-Fas Antibody (human, neutralizing), clone ZB4 分析证书

标题批号
Anti-Fas (human, neutralizing), clone ZB4 3070787
Anti-Fas (human, neutralizing), clone ZB4 - 2120009 2120009
Anti-Fas (human, neutralizing), clone ZB4 - 2135062 2135062
Anti-Fas (human, neutralizing), clone ZB4 - 2139985 2139985
Anti-Fas (human, neutralizing), clone ZB4 - 2392215 2392215
Anti-Fas (human, neutralizing), -2554838 2554838
Anti-Fas (human, neutralizing), -2577037 2577037
Anti-Fas (human, neutralizing), -2585868 2585868
Anti-Fas (human, neutralizing), -2602776 2602776
Anti-Fas (human, neutralizing), -2659141 2659141

参考

参考概述应用公共医疗ID
Networked T cell death following macrophage infection by Mycobacterium tuberculosis.
Macdonald, SH; Woodward, E; Coleman, MM; Dorris, ER; Nadarajan, P; Chew, WM; McLaughlin, AM; Keane, J
PloS one  7  e38488  2012

显示摘要
22675566 22675566
p53-mediated delayed NF-κB activity enhances etoposide-induced cell death in medulloblastoma.
Meley, D; Spiller, DG; White, MR; McDowell, H; Pizer, B; Sée, V
Cell death & disease  1  e41  2010

显示摘要
21364648 21364648
Elucidation of molecular events leading to neutrophil apoptosis following phagocytosis: cross-talk between caspase 8, reactive oxygen species, and MAPK/ERK activation.
Zhang, Bin, et al.
J. Biol. Chem., 278: 28443-54 (2003)  2003

显示摘要
12736263 12736263
Molecular determinants of apoptosis induced by the cytotoxic ribonuclease onconase: evidence for cytotoxic mechanisms different from inhibition of protein synthesis.
M S Iordanov, O P Ryabinina, J Wong, T H Dinh, D L Newton, S M Rybak, B E Magun
Cancer research  60  1983-94  2000

显示摘要
10766189 10766189
Regulation of Fas ligand expression and cell death by apoptosis-linked gene 4
Lacana, E. and D'Adamio, L.
Nat Med, 5:542-7 (1999)  1999

Neutralization Assay10229231 10229231
Stimulated human lamina propria T cells manifest enhanced Fas-mediated apoptosis
Boirivant, M., et al
J Clin Invest, 98:2616-22 (1996)  1996

Neutralization Assay8958226 8958226
Involvement of apoptosis antigen Fas in clonal deletion of human thymocytes
Yonehara, S, et al
Int Immunol, 6:1849-56 (1994)  1994

7535095 7535095