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MABN698 Anti-FABP2 Antibody, clone 9A9B7B3

Detect Fatty Acid Binding Protein using this mouse monoclonal antibody, Anti-FABP2 Antibody, clone 9A9B7B3 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.

Anti-FABP2 Antibody, clone 9A9B7B3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: FABPI, FABP2 UniProt Number: P12104
MABN698
100 µL  
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概述

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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IF, IHC, FCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABN698
ReplacesMABS390; MABS401
DescriptionAnti-FABP2 Antibody, clone 9A9B7B3
Alternate Names
  • Fatty acid-binding protein, intestinal
  • Fatty acid-binding protein 2
  • Intestinal-type fatty acid-binding protein
  • I-FABP
Background InformationFatty acid-binding protein 2, (FABP2) is a fatty acid-binding protein produced by the intestinal tissues of the small intestine and participates in the transport and intracellular internalization and metabolism of fatty acids in the gut. Its expression is localized to the small intestine, especially within the cellular epithelial cells of the jejunum, with much lower levels in the large intestine. Its expression is confined to the cytoplasm and induced by growth factors such as EGF. FABP2 is also known as FABPI or I-FABP. Because of its role in fatty acid uptake and lipid homeostasis, mutations or polymorphisms affecting the function of FABP2 in fatty acid metabolism have been a source of recent research. In particular, an amino acid substitution known as A54T where the G to A polymorphism of codon 54 of the FABP2 gene results in the substation of a Thr in the mutated form instead of an Ala in the normal form. This Thr-codon 54 mutation demonstrates an increased affinity for long-chain fatty acids and is strongly associated with factors associated with metabolic syndrome disease, including insulin resistance, enhanced fatty acid oxidation, elevated triglycerides and type 2 diabetes. Furthermore, FABPs and in particular , FABP2, play an increasingly important role in controlling the lipid signaling cascade and FABP2 is being studied as key regulatory factor of lipid signaling under normal and pathological conditions. EMD-Millipore’s anti-FABP2 mouse monoclonal antibody, clone 9A9B7B3, has been tested in western blot, paraffin embedded immunohistochemical, and flow cytometry studies using human tissues and cell lines. The clone also reacts with FABP2 from NIH-3T3-L1 mouse cells lines by immunocytochemistry.
References
Product Information
FormatAscites
Control
  • Human small intestine tissue lysate
PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Fatty Acid Binding Protein using this mouse monoclonal antibody, Anti-FABP2 Antibody, clone 9A9B7B3 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunohistochemistry
  • Flow Cytometry
Application NotesImmunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected FABP2 in 3T3-L1 cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected FABP2 in human small Intestine tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected FABP2 in LOVO cells.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified recombinant fragment of human FABP2 expressed in E. Coli.
Clone9A9B7B3
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • FABPI
  • FABP2
Purification MethodUnpurified
UniProt Number
Molecular Weight~15 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human small intestine tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected FABP2 in human small intestine tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABN698 04053252914386