数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

GR13 Sigma-AldrichAnti-EGFR (Ab-3) Mouse mAb (225)

Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation.

Anti-EGFR (Ab-3) Mouse mAb (225) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书
参考
Data Sheet

同义词: Anti- Epidermal Growth Factor Receptor

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

Products

产品目录编号	包装	数量 / 包装
GR13-100UGCN	塑胶安瓿;塑胶针药瓶	100 μg	添加到收藏夹添加到收藏夹添加到我的收藏夹

Description
Overview	Recognizes the ~170 kDa epidermal growth factor receptor protein. For neutralization studies Cat. No. GR13L is recommended.
Catalogue Number	GR13
Brand Family	Calbiochem®
Synonyms	Anti- Epidermal Growth Factor Receptor

References
References	Hunter T. 1984. Nature 311, 414. Reynolds, F.H., et. al 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.

Product Information
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative control	HL-60 cells
Positive control	A-431 cells
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Original Clone Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Masui, H., et al. 1984. Cancer Res. 44, 1002. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Biol. Med. 1, 511.
Key Applications	Immunoprecipitation
Application Notes	Immunoprecipitation (1-2 µg per sample) Neutralization Studies (2 µg/ml, see application references; use Cat. No. GR13L)
Application Comments	Shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. For neutralization studies GR13L is recommended. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	partially purified, human EGFR from A431 cells
Immunogen	Human
Clone	225
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
GR13-100UGCN	04055977228243

Documentation

Anti-EGFR (Ab-3) Mouse mAb (225) 分析证书

标题	批号
GR13	输入批号

参考

参考信息概述
Hunter T. 1984. Nature 311, 414. Reynolds, F.H., et. al 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	25-October-2007 JSW
Synonyms	Anti- Epidermal Growth Factor Receptor
Application	Immunoprecipitation (1-2 µg per sample) Neutralization Studies (2 µg/ml, see application references; use Cat. No. GR13L)
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.
Background	Epidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF receptor is phosphorylated on tyrosine residues. Subsequent to binding, EGF receptor complexes are internalized and cell proliferation takes place with partially-purified EGF receptor from human A431 epidermal carcinoma cells.
Host	Mouse
Immunogen species	Human
Immunogen	partially purified, human EGFR from A431 cells
Clone	225
Isotype	IgG₁
Species	human, not mouse, not rat
Positive control	A-431 cells
Negative control	HL-60 cells
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. For neutralization studies GR13L is recommended. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Hunter T. 1984. Nature 311, 414. Reynolds, F.H., et. al 1981. Nature 292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
Application references	Original Clone Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Masui, H., et al. 1984. Cancer Res. 44, 1002. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Biol. Med. 1, 511.

产品目录编号	描述
GR13L	Anti-EGFR (Ab-3) Mouse mAb (225)

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

此产品已添加到您的购物车。