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MABT188 Sigma-AldrichAnti-Dynamin-1/2 Antibody, clone Hudy-1

Anti-Dynamin-1/2 Antibody, clone Hudy-1 detects level of Dynamin-1/2 & has been published & validated for use in Western Blotting, Immunofluorescence.

Anti-Dynamin-1/2 Antibody, clone Hudy-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: DNM1, DNM UniProt Number: Q05193

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, H	WB, IF	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABT188
Description	Anti-Dynamin-1/2 Antibody, clone Hudy-1
Alternate Names	Dynamin-1 Dynamin Dynamin-1/2
Background Information	Dynamin-1 & Dynamin-2 belong to the dynamin superfamily of large GTPases. Dynamins are multidomain proteins consisting of a GTP-binding domain, a middle domain, and a GTP-effector domain. Dynamins also contain a pleckstrin homology (PH) domain which may facilitate membrane binding. Dynamin-1 is highly abundant in the brain particularly at synaptic contacts, whereas Dynamin-2 is ubiquitously expressed in non-neuronal cells. Dynamins are involved in receptor-mediated endocytosis of clathrin-coated vesicles. Dynamin-1 interacts with an array of cellular targets via its SH3 binding domain and is implicated in late stages of vesicle formation by forming oligomers around the “neck” of budding vesicles and inducing membrane fission; it may also play a role in cytoskeleton rearrangement and cytoskinesis. Dynamin-2 is involved in the formation of clathrin-coated vesicles but may also be involved in the recycling of receptors between endosomes and the trans-Golgi network.

References

Product Information
Format	Purified
Control	Rat brain tissue lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Dynamin-1/2 Antibody, clone Hudy-1 detects level of Dynamin-1/2 & has been published & validated for use in Western Blotting, Immunofluorescence.
Key Applications	Western Blotting Immunofluorescence
Application Notes	Immunofluorescence Analysis: A representative lot was used by an independent laboratory in HeLa cells (Nicoziani, P., et al. (1999). Molecular Biology of the Cell. 11:481–495.)

Biological Information
Immunogen	Recombinant full length human Dynamin-1 expressed in transfected Sf9 cells.
Clone	Hudy-1
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody detects both Dynamin-1 and Dynamin-2. (Nicoziani, P., et al. (1999). Molecular Biology of the Cell. 11:481–495.)
Isotype	IgG1κ
Species Reactivity	Rat Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001005336
Entrez Gene Summary	This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008].
Gene Symbol	DNM1 DNM
Purification Method	Protein G Purified
UniProt Number	Q05193
UniProt Summary	FUNCTION: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis. CATALYTIC ACTIVITY: GTP + H2O = GDP + phosphate. SUBUNIT STRUCTURE: Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3 (By similarity). Interacts with PHOCN By similarity. Interacts with SNX9. SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytoskeleton. Note: Microtubule-associated. SEQUENCE SIMILARITIES: Belongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain.
Molecular Weight	~97 kDa observed. Dynamin 1 has a calculated MW of 97 kDa. Dynamin 2 has a calcluated MW of 98 kDa.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in rat brain tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin-1/2 on 10 µg of rat brain tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABT188	04053252548925

Documentation

Anti-Dynamin-1/2 Antibody, clone Hudy-1 MSDS

职位
物料安全数据表 (MSDS)

Anti-Dynamin-1/2 Antibody, clone Hudy-1 分析证书

标题	批号
Anti-Dynamin-1/2, clone Hudy-1	3073853
Anti-Dynamin-1/2, clone Hudy-1 - 2061574	2061574
Anti-Dynamin-1/2, clone Hudy-1 - 2829145	2829145
Anti-Dynamin-1/2, clone Hudy-1 - 3202280	3202280
Anti-Dynamin-1/2, clone Hudy-1 - 3314355	3314355
Anti-Dynamin-1/2, clone Hudy-1 - 4020098	4020098
Anti-Dynamin-1/2, clone Hudy-1 - 4142790	4142790
Anti-Dynamin-1/2, clone Hudy-1 - Q1984917	Q1984917
Anti-Dynamin-1/2, clone Hudy-1 -2502608	2502608
Anti-Dynamin-1/2, clone Hudy-1 -2571280	2571280

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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