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MABT1349-25UG Anti-Desmoglein-2 Antibody, clone CSTEM28

Anti-Desmoglein-2 , clone CSTEM28, Cat. No. MABT1349, is a mouse monoclonal antibody that detects Desmoglein-2 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

MABT1349-25UG
25 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABT1349-25UG
DescriptionAnti-Desmoglein-2 Antibody, clone CSTEM28
Alternate Names
  • Cadherin family member 5
  • HDGC
Background InformationDesmoglein-2 (UniProt: Q14126; also known as Cadherin family member 5, HDGC) is encoded by the DSG2 (also known as CDHF5) gene (Gene ID: 1829) in human. Desmogleins are protein substances located in and on the surface of keratinocytes. They are present in specialized cell-cell adhesion structures enriched in tissues that undergo substantial mechanical stress. These proteins have been shown to be a critical factor in cell-to-cell adhesion. Desmoglein-2 is a single-pass type I membrane glycoprotein that is found in all tissues and in carcinomas. It serves as a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It is synthesized with a signal peptide (aa 1-23) and a propeptide (aa 24-49) that are subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 50-609), a transmembrane domain (aa 610-634), and a cytoplasmic domain (aa 635-1118). Mutations in DSG2 gene have been linked to familial type of arrhythmogenic right ventricular dysplasia that is characterized y infiltration of adipose and fibrous tissue into the right ventricle and loss of myocardial cells, resulting in ventricular and supraventricular arrhythmias. Desmoglein-2 levels are reported to be consistently upregulated in most cancers where it participates in the maintenance of intracellular junctions that prevents host immune responses. (Ref.: O Brien, CM., et al. (2017). Stem Cells. 35(3); 626-640).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Desmoglein-2 , clone CSTEM28, Cat. No. MABT1349, is a mouse monoclonal antibody that detects Desmoglein-2 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry
Application NotesTested Applications

Flow Cytometry Analysis: A representative lot detected Desmoglein-2 in Flow Cytometry (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Immunocytochemistry Analysis: A representative lot detected Desmoglein-2 in Immunocytochemistry (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

ELISA Analysis: A representative lot detected Desmoglein-2 in ELISA (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to the extracellular domain of human Desmoglein-2.
EpitopeExtracellular domain
CloneCSTEM28
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone CSTEM28 is a mouse monoclonal antibody that detects Desmoglein-2. It targets an epitope within the extracellular domain.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DSG2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~78 kDa observed for recombinant fragment, 122.29 kDa calculated for full-length protein. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant human Desmoglein-2.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human Desmoglein-2.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABT1349-25UG 04065268529058