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MAB8131 Anti-Cytomegalovirus Antibody, immediate early, clone 6F8.2

MAB8131
100 µg  
Purchase on Sigma-Aldrich

特卖

概述

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIF, WB, ICC, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB8131
ReplacesMAB8130
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Cytomegalovirus Antibody, immediate early, clone 6F8.2
Alternate Names
  • CMV
Product Information
FormatPurified
PresentationPurified immunoglobulin. Supplied in PBS buffer, pH 7.4. Contains 0.1% sodium azide and carrier protein. Has been sterile filtered.
Quality LevelMQ300
Applications
ApplicationAnti-Cytomegalovirus Antibody, immediate early, clone 6F8.2 is an antibody against Cytomegalovirus for use in IF, WB, IC, IH(P).
Key Applications
  • Immunofluorescence
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry.

Immunocytochemistry.

Western Blot 1:100

IFA at 1:600-1:1,200 on acetone fixed cells.

Works on paraffin embedded tissue sections.

Final working dilutions must be determined by end user.
Biological Information
ImmunogenAffinity purified early antigen from MRC-5 cells infected with CMV AD169 (ATCC).
Epitopeimmediate early
Clone6F8.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with an immediate early protein M.W. 68-72 kD. Can detect CMV infection 1 hour post-infection exhibiting a nuclear and/or intranuclear inclusion staining which reaches peak intensity between 8-24 hours.

With CMV the antigens expressed at different times are listed as:



Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at +4°C for up to 12 months
Packaging Information
Material Size100 µg
Global Trade Item Number
产品目录编号 GTIN
MAB8131 04053252580406