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MABE1060 Anti-CtIP Antibody, clone 14-1

Anti-CtIP Antibody, clone 14-1, Cat. No. MABE1060, is a highly specific mouse monoclonal antibody that targets CtIP and has been used in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-CtIP Antibody, clone 14-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: RBBP8, CTIP UniProt Number: Q99708
MABE1060
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, ICC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1060
DescriptionAnti-CtIP Antibody, clone 14-1
Alternate Names
  • CtBP-interacting protein
  • CtIP
  • Retinoblastoma-binding protein 8
  • RBBP-8
  • Retinoblastoma-interacting protein and myosin-like
  • RIM
  • Sporulation in the absence of SPO11 protein 2 homolog
  • SAE2
Background InformationDNA endonuclease RBBP8 (UniProt: Q99708; also known CtBP-interacting protein, CtIP, Retinoblastoma-binding protein 8, RBBP-8, Retinoblastoma-interacting protein and myosin-like, RIM, Sporulation in the absence of SPO11 protein 2 homolog, SAE2) is encoded by the RBBP8 (also known as CTIP) gene (Gene ID: 5932) in human. RBBP-8 is an endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. RBBP8 is phosphorylated upon DNA damage (at Thr859) by ATR and this phosphorylated form binds to chromatin to initiate resection. The non-phosphorylated form does not bind to chromatin. It is reported that ATM activity is required for an early step in resection, leading to ATR activation, CtIP- phosphorylation, and accumulation of CtIP on chromatin. RBBP8 functions downstream of the MRN complex and promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. It is localized predominantly in the nucleus and interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein. By interacting with BCRA1 it modulates transcriptional regulation, DNA repair, and cell cycle checkpoint control. Mutations in RBBP8 gene are known to cause Seckel syndrome 2, an autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly, and mental retardation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CtIP Antibody, clone 14-1, Cat. No. MABE1060, is a highly specific mouse monoclonal antibody that targets CtIP and has been used in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesImmunocytochemistry Analysis: A representative lot detected CtIP in Immunocytochemistry applications (Murina, O., et. al. (2014). Cell Rep. 7(4):1030-8).

Western Blotting Analysis: A representative lot detected CtIP in Western Blotting applications (Yu, X., et. al. (2000). J Biol Chem. 275(24):18541-9; Bunting, S.F., et. al. (2010). Cell. 141(2):243-54; Wu, M., et. al. (2007). Mol Cancer Res. 5(12):1285-95).

Immunoprecipitation Analysis: A representative lot was used for CtIP Immunoprecipitation. (Yu, X., et. al. (2000). J Biol Chem. 275(24):18541-49).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 278 amino acids from the C-terminal half of human CtBP-interacting protein.
Epitopedomain of:
Clone14-1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 14-1 specifically detects CtBP-interacting protein in HEK293 cells.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RBBP8
  • CTIP
Purification MethodProtein G purified
UniProt Number
Molecular Weight~120 kDa observed; 101.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.


Western Blotting Analysis: 0.5 µg/mL of this antibody detected CtIP in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABE1060 04054839168765