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MABF2239-100UL Anti-Complement receptor 1 Antibody, clone 7G9

MABF2239-100UL
100 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABF2239-100UL
DescriptionAnti-Complement receptor 1 Antibody, clone 7G9
Alternate Names
  • C3b/C4b receptor
  • CD35
Background InformationComplement receptor type 1 (UniProt: P17927; also known as C3b/C4b receptor, CD35, CR1) is encoded by the CR1 (also known as C3BR) gene (Gene ID: 1378) in human. CR1 is a single-pass type I membrane glycoprotein that is expressed in most peripheral blood cells, follicular-dendritic cells, Schwann cells, and glomerular podocytes. It regulates the activation of the complement cascade by serving as a cofactor for the factor I-mediated cleavage of C3b and C4b and also accelerates the decay of the C3 and C5 convertases. It is shown to bind to C3b and C4b opsonized immune complex on erythrocytes and transports them to liver and spleen where they are degraded by phagocytes. CR1 inhibits spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d. It also plays a role in immune regulation by contributing to the generation of regulatory T cells from activated helper T cells. CR1 is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to generate the mature form with a long extracellular domain (aa 42-1971), a short transmembrane domain (aa 1972-1996) and a cytoplasmic tail (aa 1997-2039). (Ref.: Nickells, M., et al. (1988). Clin. Exp. Immunol. 112(1); 27-33; Reist, CJ et al. (1994). Eur. J. Immunol. 24(9); 2018-2025).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Complement receptor 1, clone 7G9, Cat. No. MABF2239, is a mouse monoclonal antibody that detects Complement receptor 1 and has been tested for use in Dot Blot, Flow Cytometry, and Radioimmunoassay.
Key Applications
  • Dot Blot
  • Flow Cytometry
  • Radioimmunoassay
Application NotesDot Blot Analysis: A representative lot detected Complement receptor 1 in Dot Blot applications (Nickells, M., et. al. (1998). Clin Exp Immunol. 112(1):27-33).

Radioimmunoassay Analysis: A representative lot detected Complement receptor 1 in Radioimmunoassay applications (Hahn, C.S., et. al. (2001). J Immunol. 166(2):1057-65).
Biological Information
ImmunogenSoluble human complement receptor 1.
Epitopeextracellular domain
Clone7G9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 7G9 is a mouse monoclonal antibody that detects human complement receptor 1 (CR1). it binds to long homologous repeats (LHR) A,B,C of CR1.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Rhesus Macaque
Species Reactivity NoteHuman, Rhesus Monkey.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CR1
  • C3BR
Purification MethodProtein G purified
UniProt Number
Molecular Weight223.66 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in TF-1 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected Complement receptor 1 in one million TF-1 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABF2239-100UL 04054839990625

Documentation

Anti-Complement receptor 1 Antibody, clone 7G9 MSDS

职位

物料安全数据表 (MSDS) 

Anti-Complement receptor 1 Antibody, clone 7G9 分析证书

标题批号
Anti-Complement receptor 1, clone 7G9 - 4074812 4074812
Anti-Complement receptor 1, clone 7G9 - Q3224770 Q3224770

参考

参考概述公共医疗ID
Bispecific monoclonal antibodies mediate binding of dengue virus to erythrocytes in a monkey model of passive viremia.
Hahn, CS; French, OG; Foley, P; Martin, EN; Taylor, RP
J Immunol  166  1057-65  2001

显示摘要
11145685 11145685
Mapping epitopes for 20 monoclonal antibodies to CR1.
Nickells, M; Hauhart, R; Krych, M; Subramanian, VB; Geoghegan-Barek, K; Marsh, HC; Atkinson, JP
Clin Exp Immunol  115  27-36  2001

显示摘要
9566786 9566786