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MAB1254 Anti-Chymase Antibody, clone B7

MAB1254
100 µg  
Purchase on Sigma-Aldrich

特卖

概述

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HDB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1254
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Chymase Antibody, clone B7
Alternate Names
  • Mast Cell Protease I
Background InformationMast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.
Product Information
FormatPurified
Control
  • POSITIVE CONTROL: lung, heart, skin, placenta.
PresentationPurified by protein G affinity chromatography. Liquid in PBS with 20% glycerol, pH 7.4.
Quality LevelMQ100
Applications
ApplicationAnti-Chymase Antibody, clone B7 is an antibody against Chymase for use in DB, IH.
Key Applications
  • Dot Blot
  • Immunohistochemistry
Applications Not Recommended
  • Western Blotting
Application NotesDot blotting: 0.5-1.0 μg/mL.

Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy's Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

Not recommended for use on Western blots.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenChymase purified from human skin.
CloneB7
Concentration2.4 mg/mL
HostMouse
SpecificityReacts with human mast cell chymase.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases.
Gene Symbol
  • CMA1
  • CYM
  • MGC119890
  • Alpha-chymase
  • MCT1
  • MGC119891
  • CYH
  • EC 3.4.21.39
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P23946 # Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
SIZE: 247 amino acids; 27325 Da
SUBCELLULAR LOCATION: Secreted. Cytoplasmic granule. Note=Mast cell granules.
TISSUE SPECIFICITY: Mast cells in lung, heart, skin and placenta.
SIMILARITY: SwissProt: P23946 ## Belongs to the peptidase S1 family. Granzyme subfamily. & Contains 1 peptidase S1 domain.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. Antibody may also be stored at -20ºC in undiluted aliquots.
Packaging Information
Material Size100 µg
Global Trade Item Number
产品目录编号 GTIN
MAB1254 04053252465949