数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

ABN458 Sigma-AldrichAnti-Choline Transporter Antibody

Anti-Choline Transporter, Cat. No. ABN458, is a rabbit polyclonal antibody that detects Choline Transporter and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Choline Transporter Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: SLC5A7, CHT1 UniProt Number: Q9GZV3

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
R	WB, IH(P)	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABN458
Replaces	AB5960;AB5966
Description	Anti-Choline Transporter Antibody
Alternate Names	High affinity choline transporter 1 Hemicholinium-3-sensitive choline transporter CHT Solute carrier family 5 member 7
Background Information	High affinity choline transporter 1 (UniProt: Q9GZV3; also known as Hemicholinium-3-sensitive choline transporter, CHT, Solute carrier family 5 member 7) is encoded by the SLC5A7 (also known as CHT1) gene (Gene ID: 60482) in human. CHT is a multi-pass membrane protein with six extracellular domains, eleven transmembrane domains, and six cytoplasmic domains. It is expressed in cholinergic neurons in putamen, spinal cord, and medulla and serves as a transmembrane transporter that imports choline from the extracellular space into neurons with high affinity in a sodium and chloride ion-dependent manner. In neurons it is acetylated by the action of choline acetyltransferase to generate acetylcholine. Choline uptake is shown to be the rate-limiting step in acetylcholine synthesis. The majority of CHT is localized intracellularly including a proportion present on acetylcholine-containing synaptic vesicles. Diminished levels of acetylcholine in selected areas of the nervous system are linked to several neurodegenerative diseases. Mutation in SLC5A7 gene have been linked to neuronopathy and myasthenic syndrome that is characterized by failure of neuromuscular transmission, fatigability, and muscle atrophy and weakness. (Ref.: Bauche, S., et al. (2016). Am. J. Hum. Genet. 99(3); 753-761; Barwick, KES., et al. (2012). Am. J. Hum. Genet. 91(6); 1103-1107).

References

Product Information
Format	Affinity Purified
HS Code	3002 15 90
Presentation	Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality Level	MQ100

Applications
Application	Anti-Choline Transporter, Cat. No. ABN458, is a rabbit polyclonal antibody that detects Choline Transporter and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications	Western Blotting Immunohistochemistry (Paraffin)
Application Notes	Tested Applications Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Choline Transporter in rat spinal cord and human cerebral cortex tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Biological Information
Immunogen	GST-tagged recombinant fragment corresponding to 77 amino acids from the C-terminal region of human High affinity choline transporter.
Epitope	C-terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This rabbit polyclonal antibody detects high affinity choline transporter. It targets an epitope within the C-terminal region.
Species Reactivity	Rat
Species Reactivity Note	Rat. Predicted to react with Human, Mouse based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_068587
Gene Symbol	SLC5A7 CHT1
Purification Method	Affinity Purfied
UniProt Number	Q9GZV3
Molecular Weight	~63 kDa observed; 63.2 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in Rat spinal cord tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Choline Transporter in Rat spinal cord tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at +2°C to +8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ABN458	04053252941436

Documentation

Anti-Choline Transporter Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-Choline Transporter Antibody 分析证书

标题	批号
Anti-Choline Transporter - Q2343010	Q2343010
Anti-Choline Transporter - 3218873	3218873
Anti-Choline Transporter - 3288744	3288744
Anti-Choline Transporter - 3474173	3474173
Anti-Choline Transporter - 3745922	3745922
Anti-Choline Transporter - 3783265	3783265
Anti-Choline Transporter - 3857741	3857741
Anti-Choline Transporter - 3861356	3861356
Anti-Choline Transporter - 3956639	3956639
Anti-Choline Transporter - 4050752	4050752

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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