Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

07-1493 Anti-Calcineurin Aβ Antibody

Detect Calcineurin Aβ using this Anti-Calcineurin Aβ Antibody validated for use in WB, IH.

Anti-Calcineurin Aβ Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: GPCR, cAMP/cGMP & Calcium Signaling Gene Symbol: MGC138448, NF-ATC , NF-ATc , NF-ATc1 , NFAT2 , NFATC, NFATc UniProt Number: O95644
07-1493
100 µL  
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ma, HWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1493
ReplacesAB1697
DescriptionAnti-Calcineurin Aβ Antibody
Alternate Names
  • NFAT transcription complex cytosolic component
  • nuclear factor of activated T-cells, cytoplasmic,
    calcineurin-dependent 1
  • nuclear factor of activated T-cells, cytosolic component 1
Background InformationCalcineurin is a serine/threonine protein phosphatase involved in the mediation of signal transduction in a wide variety of cellular responses. Calcineurin has been linked to Pmk1 MAP kinase and PI pathways, as well as to members of the small GTPase Rab/Ypt family and Type II myosin. Furthermore, calcineurin participates in the transduction of extracellular signals to the nucleus by dephosphorylation of members of the NFAT family of transcription factors. These linkages demonstrate calcineurin's multifunctional capabilities in the regulation of calcium signaling. The Aα form of calcineurin is a major catalytic subunit gene isoform of calcineurin in most tissues that allows differentiation of Aα from the Aβ and Aγ isoforms.
References
Product Information
FormatAffinity Purified
Control
  • Human brain lysate
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 40% gylerol. Contains no preservative.
Quality LevelMQ100
Applications
ApplicationDetect Calcineurin Aβ using this Anti-Calcineurin Aβ Antibody validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Biological Information
ImmunogenKLH-conjugagted synthetic peptide corresponding to Calcineurin Aβ.
HostRabbit
SpecificityRecognizes the Aβ (polyproline) form of calcineurin, a less abundant gene isoform of calcineurin in most tissues. Allows differentiation of Aβ from the A α and A-γ isoforms. Reacts with calcineurin of all mammalian species.
Species Reactivity
  • Mammals
  • Human
Species Reactivity NoteProven to react with human. Predicted to react with all mammals based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MGC138448
  • NF-ATC
  • NF-ATc
  • NF-ATc1
  • NFAT2
  • NFATC
  • NFATc
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.
SUBUNIT STRUCTURE: Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
TISSUE SPECIFICITY: Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.
INDUCTION: Only isoforms A are inducibly expressed in T lymphocytes upon activation of the T-cell receptor (TCR) complex. Induced after co-addition of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Also induced after co-addition of 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin. Weakly induced with PMA, ionomycin and cyclosporin A.
DOMAIN: Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.
Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.
PTM: Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
SEQUENCE SIMILARITIES: Contains 1 RHD (Rel-like) domain.
Molecular WeightApprox. 58 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human brain lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Calcineurin Aβ on 10 µg of human brain lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C for up to 12 months in undiluted aliquots; at this temperature the solution will
remain unfrozen. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
07-1493 04053252319952

Documentation

Anti-Calcineurin Aβ Antibody MSDS

职位

物料安全数据表 (MSDS) 

Anti-Calcineurin Aβ Antibody 分析证书

标题批号
Anti-Calcineurin A - 2277885 2277885
Anti-Calcineurin A - NG1905696 NG1905696
Anti-Calcineurin A - NRG1693940 NRG1693940
Anti-Calcineurin A#946; - 2147101 2147101
Anti-Calcineurin A#946; - 1956742 1956742
Anti-Calcineurin A#946; - 2035079 2035079
Anti-Calcineurin Aβ - 2330624 2330624
Anti-Calcineurin Aβ - 3313322 3313322
Anti-Calcineurin Aβ - 3385737 3385737
Anti-Calcineurin Aβ - 3695824 3695824
Show more

参考

参考概述应用公共医疗ID
Calcineurin regulates nuclear factor I dephosphorylation and activity in malignant glioma cell lines.
Brun, M; Glubrecht, DD; Baksh, S; Godbout, R
The Journal of biological chemistry  288  24104-15  2013

显示摘要 		Western Blotting23839947 23839947