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MABC1207 Anti-CIRP (CIRBP) Antibody, clone 6C7.1

Detect CIRBP using this mouse monoclonal Anti-CIRP (CIRBP) Antibody, clone 6C7.1, Cat. No. MABC1207, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-CIRP (CIRBP) Antibody, clone 6C7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: CIRBP, A18HNRNP, CIRP UniProt Number: Q14011
MABC1207
100 µL  
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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RIH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1207
DescriptionAnti-CIRP (CIRBP) Antibody, clone 6C7.1
Alternate Names
  • Cold-inducible RNA-binding protein
  • A18 hnRNP
  • Glycine-rich RNA-binding protein CIRP
Background InformationCold-inducible RNA-binding protein (UniProt Q14011; also known as A18 hnRNP, Glycine-rich RNA-binding protein CIRP) is encoded by the CIRBP (also known as A18HNRNP, CIRP) gene (Gene ID 1153) in human. CIRP is an 18-kDa RNA-binding protein with an RNA recognition motif (RRM; a.a. 6-84) and a glycine-rich domain (a.a. 92-158). CIRP migrates from nucleus to cytoplasm, where it mediates posttranscriptional regulation of target mRNAs. Originally characterized and named as a cold-induced protein, CIRP exerts antiapoptotic and cell-protective effects in response to cold stress, as well as other stimuli such as UV irradiation and hypoxia. CIRP is expressed in a broad range of tissues (e.g. testis, brain, lung, kidney, liver, stomach, bone marrow, and heart) and upregulated CIRP levels have been reported in several types of cancers. CIRP knockdown is reported to increase p53 level and enhance cancer cells chemo-sensitivity to DNA damage inducing drugs. Likewise, CIRP overexpression results in p53 downregulation.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect CIRBP using this mouse monoclonal Anti-CIRP (CIRBP) Antibody, clone 6C7.1, Cat. No. MABC1207, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected CIRP (CIRBP) in rat testis and human prostate cancer, pancreas, tonsil tissue sections.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected CIRP (CIRBP) in 10 µg of Raji human B-cell lymphoma cell lysate.
Biological Information
ImmunogenGST-tagged recombinant human CIRP (CIRBP) internal fragment.
Clone6C7.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 6C7.1 is expected to recognize CIRP spliced isoforms 1 and 3 (UniProt Q14011). Cross-reactivity toward spliced isoform 2 is possible, but has not been confirmed.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CIRBP
  • A18HNRNP
  • CIRP
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~18 kDa observed. 18.65/28.36/26.13 kDa (isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected CIRP (CIRBP) in 10 µg of SH-SY5Y human neuroblastoma cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABC1207 04054839069628