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ABN1646 Anti-CDC42 Antibody, Brain Isoform-specific

Anti-CDC42, Brain Isoform-specific, Cat. No. ABN1646, is a highly specific rabbit polyclonal antibody that targets CDC42 and has been tested in Immunocytochemistry and Western Blotting.

Anti-CDC42 Antibody, Brain Isoform-specific MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: CDC42 UniProt Number: P60953-1
ABN1646
100 μg  
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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RICC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1646
DescriptionAnti-CDC42 Antibody, Brain Isoform-specific
Alternate Names
  • Cell division control protein 42 homolog
  • G25K GTP-binding protein
Background InformationCell division control protein 42 homolog (UniProt: P60953; also known as G25K GTP-binding protein) is encoded by the CDC42 gene (Gene ID 998) in human. CDC42 homolog is a plasma membrane-associated small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state. Its activity is regulated by guanine nucleotide exchange factors (GEFs), which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) that increase the GTP hydrolysis activity, and GDP dissociation inhibitors, which inhibit the dissociation of the nucleotide from the GTPase. In the active state it binds to a variety of effector proteins to regulate cellular responses. Cdc42 is known to regulate the dynamic organization of the cytoskeleton and membrane trafficking for physiologic processes such as cell proliferation, motility, polarity, cytokinesis and growth. Deregulation of Cdc42 is found in several pathogenic processes such as cancer, cardiovascular diseases, and neuronal degenerative diseases. It is involved in epithelial cell polarization processes and regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. During the prometaphase stage, it localizes to spindle and moves to the central spindle as cells progress through anaphase to telophase.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-CDC42, Brain Isoform-specific, Cat. No. ABN1646, is a highly specific rabbit polyclonal antibody that targets CDC42 and has been tested in Immunocytochemistry and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected GFP-tagged recombinant CDC42 brain isoform (Cdc42-palm), but not the placenta isoform Cdc42-prenyl exogenously expressed in COS-7 cells (Courtesy provided by Rujun).

Immunocytochemistry Analysis: A representative lot detected CDC42 brain isoform (Cdc42-palm) at the axon tip by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized DIV3cortical neurons from Zdhhc8-deficient mice (Mukai, J., et al. (2015). Neuron. 86(3):680-695).

Western Blotting Analysis: A representative lot detected CDC42 brain isoform (Cdc42-palm) in whole cell lysate and among purified palmitoylated proteins (PPs) from cultured rat cortical embryonic neurons (Kang, R., et al. (2008). Nature. 456(7224):904-909).

Western Blotting Analysis: A representative lot detected CDC42 brain isoform (Cdc42-palm) in rat brain membrane fractions (Kang, R., et al. (2008). Nature. 456(7224):904-909).
Biological Information
ImmunogenSynthetic peptide corresponding to the C-terminal end sequence of mature human CDC42 brain isoform.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody is purified from an antiserum that has been shown to specifically detect recombinant human CDC42 brain isoform Cdc42-palm (UniProt P60953-1) with or without C188S mutation, but not the placenta isoform Cdc42-prenyl (UniProt P60953-2) by Western blotting analysis (Kang, R., et al. (2008). Nature. 456(7224):904-909). Note that the designation of CDC42 isoform 1 and 2 by Kang et al. (2008) is the exact opposite of that by UniProt P60953 (human) and P60766 (mouse), while both Kang et al. (2008) and UniProt Q8CFN2 (rat) designate the rat CDC42 brain isoform as isoform 2.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CDC42
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~21 kDa observed. 20.95/20.95/20.92 kDa (human/mouse/rat mature CDC42 brain isoform; propeptide a.a. 189-191 removed) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse hippocampus tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected CDC42 brain isoform (Cdc42-palm) in 10 µg of mouse hippocampus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABN1646 04054839112317