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MABF410 Anti-CD28 Antibody (human), PE-Cy7, clone CD28.2

This mouse monoclonal antibody Anti-CD28 (human), PE-Cy7, clone CD28.2 validated for use in IP, IHC & Flow Cytometry for the detection of CD28.

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Research Category: Inflammation & Immunology Gene Symbol: CD28 UniProt Number: P10747
MABF410
100 tests  
Purchase on Sigma-Aldrich

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概述

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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIP, IHC, FCMPE-Cy7Monoclonal Antibody
Description
Catalogue NumberMABF410
DescriptionAnti-CD28 Antibody (human), PE-Cy7, clone CD28.2
Alternate Names
  • T-cell-specific surface glycoprotein CD28
  • TP44
  • CD antigen CD28
Background InformationThe CD28.2 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICCOS and BTLA.The CD28.2 antibody may be used as a phenotypic marker for human CD28, expressed on all CD4+ T cells and CD8+ T cells, and is widely used as a reagent for activation of the CD28 receptor in vitro and in vivo. This antibody is also reported to be cross-reactive with several non-human species, including Baboon, Chimpanzee, Cynomolgus, and Rhesus.
Product Information
FormatPE-Cy7
PresentationPurified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal antibody Anti-CD28 (human), PE-Cy7, clone CD28.2 validated for use in IP, IHC & Flow Cytometry for the detection of CD28.
Key Applications
  • Immunoprecipitation
  • Immunohistochemistry
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneCD28.2
Concentration5 µL (0.25 µg)/test
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CD28
Purification MethodSize Exclusion Chromatography
UniProt Number
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD28 in one million human peripheral blood lymphocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size100 tests
Global Trade Item Number
产品目录编号 GTIN
MABF410 04053252960024