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MABC1703-25UG Anti-CCL22/MDC Antibody, clone 6F5.7

Anti-CCL22/MDC, clone 6F5.7, Cat. No. MABC1703, is a mouse monoclonal antibody that detects MDC-CCL22 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

MABC1703-25UG
25 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberMABC1703-25UG
DescriptionAnti-CCL22/MDC Antibody, clone 6F5.7
Alternate Names
  • C-C motif chemokine 22
  • CC chemokine STCP-1
  • MDC(1-69)
  • Macrophage-derived chemokine
  • Small-inducible cytokine A22
  • Stimulated T-cell chemotactic protein 1
Background InformationC-C motif chemokine 22 (UniProt: O00626; also known as CC chemokine STCP-1, MDC(1-69), Macrophage-derived chemokine, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1, CCL22) is encoded by the CCL22 (also known as MDC, SCYA22, A-152E5.1) gene (Gene ID: 6367) in human. MDC/CCL22 is a member of the intercrine beta family that plays a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. It is highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. It is reported to be chemotactic for monocytes, dendritic cells, and natural killer cells. It displays mild chemoattractant activity for primary activated T-lymphocytes but is a potent chemoattractant for chronically activated T-lymphocytes. Its signaling activity is achieved through the C-C chemokine receptor type 4 (CCR4). It acts as a mediator of lung inflammation after hemorrhage and resuscitation. MDC/CCL22 is synthesized with a signal peptide (aa 1-24) that is subsequently cleaved off. It can be proteolytically cleaved into three processed forms that are known as MDC (3-69); MDC (5-69); and MDC (7-69) (numbered without the signal peptide). (Ref.: Ritcher, JR., et al. (2014). Shock. 42(6); 525-531).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-CCL22/MDC, clone 6F5.7, Cat. No. MABC1703, is a mouse monoclonal antibody that detects MDC-CCL22 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected CCL22/MDC in human skin and human small intestine tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenRecombinant fragment of human CCL22 corresponding to 67 amino acids in the mature protein.
EpitopeUnknown
Clone6F5.7
Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 6F5.7 is a mouse monoclonal antibody that detects human CC-motif chemokine 22 (CCL22).
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CCL22
  • MDC
  • SCYA22
  • A-152E5.1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~10.0 kDa observed; 10.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant MDC-CCL22 protein.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected human recombinant MDC-CCL22 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABC1703-25UG 04065265085915