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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

05-449-C Sigma-AldrichAnti-Bin1, clone 99D (Ascites Free) Antibody

This Anti-Bin1, clone 99D (Ascites Free) Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunoprecipitation and Flow Cytometry and Immunofluorescence for the detection of Bin1.

Anti-Bin1, clone 99D (Ascites Free) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: BIN1, AMPHL UniProt Number: O00499

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Ch, M	WB, IHC, IP, FC, IF	M	Purified	Monoclonal Antibody

Description
Catalogue Number	05-449-C
Description	Anti-Bin1, clone 99D (Ascites Free) Antibody
Alternate Names	Myc box-dependent-interacting protein 1 Amphiphysin II Amphiphysin-like protein Box-dependent myc-interacting protein 1 Bridging integrator 1 Bin1
Background Information	Bin1, also known as Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, and encoded by the gene BIN1/AMPHL, is another protein involved in the regulation of synaptic vesicle endocytosis and interestingly also acts as tumor suppressor and inhibits malignant cell transformation. BIN1 is expressed in many tissues but has the highest expression in brain and muscle. BIN1 has isoforms and particular isoforms are expressed in particular tissues. For instance BIN1 isoform 11A is only expressed in the brain and found in axon initial segments and nodes of Ranvier. Mutations in BIN1 are associated with a disordered called centronuclear 2 myopathy. BIN1 is localized to nucleus but some isoforms are associated with cytoplasm.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-Bin1, clone 99D (Ascites Free) Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunoprecipitation and Flow Cytometry and Immunofluorescence for the detection of Bin1.
Key Applications	Western Blotting Immunohistochemistry Immunoprecipitation Flow Cytometry Immunofluorescence
Application Notes	Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Bin1 in human cerebellum and human skeletal muscle myocytes tissue. Western Blotting Analysis: A representative lot detected Bin1 in primary chicken embryo fibroblasts (Telfer, J.F., et al. (2005). Cellular Signalling. 17:701-8). Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63). Western Blotting Analysis: A representative lot detected Bin1 in human and rodent cell lines (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63). Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in co-immunoprecipitation studies (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77). Immunohistochemistry Analysis: A representative lot detected Bin1 in breast and liver carcinoma tissues (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77). Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75). Western Blotting Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75). Flow Cytometry Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75). Immunofluorescence Analysis: A representative lot detected Bin1 in C2C12 cells (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).

Biological Information
Immunogen	Linear peptide corresponding to human Bin1.
Clone	99D
Concentration	1 mg/mL
Host	Mouse
Isotype	IgG2bκ
Species Reactivity	Human Chicken Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_004296.1
Gene Symbol	BIN1 AMPHL
Purification Method	Protein G Purified
UniProt Number	O00499
Molecular Weight	~60 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in human skeletal muscle tissue lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Bin1 in 10 µg of human skeletal muscle tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
05-449-C	04053252978746

Documentation

Anti-Bin1, clone 99D (Ascites Free) Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-Bin1, clone 99D (Ascites Free) Antibody 分析证书

标题	批号
Anti-Bin1, clone 99D (Ascites Free) - Q2435642	Q2435642
Anti-Bin1, clone 99D (Ascites Free) - 3299962	3299962
Anti-Bin1, clone 99D (Ascites Free) - 3538142	3538142
Anti-Bin1, clone 99D (Ascites Free) - 3956980	3956980
Anti-Bin1, clone 99D (Ascites Free) -2707213	2707213
Anti-Bin1, clone 99D (Ascites Free) Monoclonal Antibody	3137648
Anti-Bin1, clone 99D (Ascites Free) Monoclonal Antibody	2979478

产品目录编号	描述
05-449	Anti-Bin1 Antibody, clone 99D
ABT21	Anti-Amphiphysin II Antibody

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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