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MABS1247 Anti-BIG1 Antibody, clone 102

Detect BIG1 using this mouse monoclonal Anti-BIG1 Antibody, clone 102, Cat. No. MABS1247. It has been tested in Immunocytochemistry and Western Blotting.

Anti-BIG1 Antibody, clone 102 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: ARFGEF1, ARFGEP1, BIG1 UniProt Number: Q9Y6D6
MABS1247
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1247
DescriptionAnti-BIG1 Antibody, clone 102
Alternate Names
  • Brefeldin A-inhibited guanine nucleotide-exchange protein 1
  • Brefeldin A-inhibited GEP 1
  • ADP-ribosylation factor guanine nucleotide-exchange factor 1
  • p200 ARF guanine nucleotide exchange factor
  • p200 ARF-GEP1
Background InformationBrefeldin A-inhibited guanine nucleotide-exchange protein 1 (UniProt: Q9Y6D6; also known as BIG1, Brefeldin A-inhibited GEP 1, ADP-ribosylation factor guanine nucleotide-exchange factor 1, p200 ARF guanine nucleotide exchange factor, p200 ARF-GEP1) is encoded by the ARFGEF1 (also known as ARFGEP1, BIG1) gene (Gene ID: 10565) in human. BIG1 is expressed in placenta, lung, heart, brain, kidney and pancreas. BIG1 activates ADP-ribosylation factor (ARF) GTPases and is involved in vesicular trafficking. It promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. BIG1 is required for the maintenance of Golgi structure and this function may be independent of its GEF activity. BIG1 and BIG2 display about 74% homology in their amino acid sequence and about 90% identity in their Sec7 domains. BIG1 is phosphorylated by PKA that reduces its GEF activity. It is localized in the cytoplasm in a multiprotein complex along with BIG2. Its nuclear localization has also been reported and it contains a nuclear localization sequence (aa 711-715). In serum starved cells treated with FK506, BIG1 is essentially completely concentrated in nuclei. BIG1 is shown to be a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity. Here BIG1 and RhoA compete with each other for the binding to myosin IXb, thus resulting in the inhibition of the GAP activity by BIG1. (Ref.: Lin, S et al. (2013). Arterioscler Thromb Vasc Biol. 33(2): e31-e38).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect BIG1 using this mouse monoclonal Anti-BIG1 Antibody, clone 102, Cat. No. MABS1247. It has been tested in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 0.5 µg/mL of this antibody detected BIG1 in 10 µg of A431 and HEK293 cell lysates.

Immunocytochemistry Analysis: A representative lot detected BIG1 in Immunocytochemistry applications (Lowery, J., et. al. (2013). J Biol Chem. 288(16):11532-45).

Western Blotting Analysis: A representative lot detected BIG1 in Western Blotting applications (Lowery, J., et. al. (2013). J Biol Chem. 288(16):11532-45).
Biological Information
ImmunogenGST-tagged recombinat fragment corresponding to 311 amino acids from human Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1).
Clone102
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 102 specifically detects BIG1 protein in human and targets an epitope within amino acids 212-522 in the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ARFGEF1
  • ARFGEP1
  • BIG1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~209 kDa observed; 208.77 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected BIG1 in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABS1247 04054839174476