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数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MAB1501X Sigma-AldrichAnti-Actin Antibody, clone C4, Alexa Fluor® 488 conjugated

Anti-Actin Antibody, clone C4, Alexa Fluor 488 conjugated detects level of Actin & has been published & validated for use in IF.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子

Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: ACTA1, MPFD, ASMA, NEM1, CFTDM, ACTA, CFTD, NEM2, Alpha-actin-1, CFTD1, NEM3 UniProt Number: P68133, P62736, P60709, P63261, P63267, P68032

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概述

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Ch	IF	M	AlexaFluor®488	Monoclonal Antibody

Description
Catalogue Number	MAB1501X
Replaces	MAB1501X
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Actin Antibody, clone C4, Alexa Fluor® 488 conjugated
Alternate Names	MAB1501 MAB1501R
Background Information	Actins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Beta- and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. It has been shown that under calcium-bound conditions, beta-actin exhibits more dynamic behavior than gamma-actin with higher rates of polymerization and depolymerization. Also, beta- and gamma-actins can readily copolymerize, and the resulting filaments exhibit polymerization and depolymerization rates that vary depending on the ratio of beta- to gamma-actin (Lessard, JL., et al (1988). Cell Motility Cytoskeleton 10(3); 349-362).

References

Product Information
Format	AlexaFluor®488
Control	HeLa cells
Presentation	Purified in PBS with 15 mg/mL BSA and 0.05% NaN3, conjugated to Alexa Fluor 488.
Quality Level	MQ100

Applications
Application	Anti-Actin Antibody, clone C4, Alexa Fluor 488 conjugated detects level of Actin & has been published & validated for use in IF.
Key Applications	Immunofluorescence
Application Notes	Immunofluorescence: 1:200-1:1000 Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Purified chicken gizzard actin (Lessard, 1988).
Epitope	Epitope is conserved in all known actins.
Clone	C4
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	MAB1501X is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts. Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
Isotype	IgG₁κ
Species Reactivity	Human Chicken
Species Reactivity Note	Reacts with human. Expected to react with chicken based on 100% homology. Other species have not been tested.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001100.3
Entrez Gene Summary	Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Gene Symbol	ACTA1 MPFD ASMA NEM1 CFTDM ACTA CFTD NEM2 Alpha-actin-1 CFTD1 NEM3
Purification Method	Protein G Purified
UniProt Number	P68133 P62736 P60709 P63261 P63267 P68032
UniProt Summary	FUNCTION: SwissProt: P68133 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. SIZE: 377 amino acids; 42051 Da SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. DISEASE: SwissProt: P68133 # Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. & Defects in ACTA1 are a cause of congenital myopathy with excess of thin myofilaments (CM) [MIM:102610]. & Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. SIMILARITY: SwissProt: P68133 ## Belongs to the actin family. MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Molecular Weight	~43 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluatied on a representative lot by immunofluorescence using HeLa cells.
Usage Statement	Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluatied on a representative lot by immunofluorescence using HeLa cells.

Usage Statement

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MAB1501X	04053252665899

Documentation

Anti-Actin Antibody, clone C4, Alexa Fluor® 488 conjugated MSDS

职位
物料安全数据表 (MSDS)

Anti-Actin Antibody, clone C4, Alexa Fluor® 488 conjugated 分析证书

标题	批号
Anti-Actin, Clone C4 Alexa Fluor® 488 conjugated - 3307307	3307307
Anti-Actin, Clone C4 Alexa Fluor® 488 conjugated - 3425562	3425562
Anti-Actin, Clone C4 Alexa Fluor® 488 conjugated - 3487584	3487584
Anti-Actin, Clone C4 Alexa Fluor® 488 conjugated - 3540407	3540407
Anti-Actin, Clone C4 Alexa Fluor® 488 conjugated - 3683851	3683851
Anti-Actin, Clone C4 Alexa Fluor® 488 conjugated - 3783276	3783276
Anti-Actin, Clone C4 Alexa Fluor® 488 conjugated - 3978180	3978180
Anti-Actin, clone C4 Alexa Fluor#174; 488 conjugated - 2115976	2115976
Anti-Actin, clone C4 Alexa Fluor#174; 488 conjugated - 2145945	2145945
Anti-Actin, clone C4 Alexa Fluor® 488 conjugated - 2379365	2379365

参考

参考概述	公共医疗ID
The Axl receptor tyrosine kinase confers an adverse prognostic influence in pancreatic cancer and represents a new therapeutic target. Koorstra JB, Karikari CA, Feldmann G, Bisht S, Rojas PL, Offerhaus GJ, Alvarez H, Maitra A Cancer Biol Ther 8 618-26. Epub 2009 Apr 22. 2009 显示摘要 BACKGROUND: Pancreatic cancer is a near uniformly lethal disease and a better understanding of the molecular basis of this malignancy may lead to improved therapeutics. The Axl receptor tyrosine kinase is implicated in cellular transformation and tumor progression, although its role in pancreatic cancer has not been previously documented. 全文本文章	19252414

参考概述

公共医疗ID

小册子

标题
AXIS: Axon Investigation System

产品目录编号	描述
MAB1501	Anti-Actin Antibody, clone C4
MAB1501R	Anti-Actin Antibody,clone C4

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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