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AB9034-50UL Anti-Acid Sensitive Ion Channel 1 Antibody

Anti-Acid Sensitive Ion Channel 1 Antibody detects level of Acid Sensitive Ion Channel 1 & has been published & validated for use in WB.

Anti-Acid Sensitive Ion Channel 1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters, Neuroinflammation & Pain Gene Symbol: ACCN2, BNaC2, ASIC1A, hBNaC2, ASIC1, ASIC, BNAC2 UniProt Number: P78348
AB9034-50UL
50 µL  
Purchase on Sigma-Aldrich

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Replacement Information

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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB9034-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Acid Sensitive Ion Channel 1 Antibody
Alternate Names
  • ASIC1
References
Product Information
FormatAffinity Purified
Control
  • Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality LevelMQ100
Applications
ApplicationAnti-Acid Sensitive Ion Channel 1 Antibody detects level of Acid Sensitive Ion Channel 1 & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern blot: 1:200 using ECL on rat brain membrane proteins.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide from rat ASIC1 (Accession P55926).The immunogen sequence is identical in human and 19/20 residues identical in mouse.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityASIC1 (Amiloride-sensitive brain sodium channel, BnaC2, ACCN2)
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Alternative splicing of this gene generates 2 transcript products.
Gene Symbol
  • ACCN2
  • BNaC2
  • ASIC1A
  • hBNaC2
  • ASIC1
  • ASIC
  • BNAC2
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P78348 # Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear (By similarity).
SIZE: 528 amino acids; 59967 Da
SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM and ACCN1 (By similarity). Interacts with PRKCABP.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Note=Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4 (By similarity).
TISSUE SPECIFICITY: Expressed in most or all neurons.
PTM: Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.
SIMILARITY: SwissProt: P78348 ## Belongs to the amiloride-sensitive sodium channel family.
MISCELLANEOUS: Potentiated by Ca(2+), Mg(2+), Ba(2+) and multivalent cations. Inhibited by anti-inflammatory drugs like salicylic acid (By similarity). Potentiated by FMRFamide-related neuropeptides. PH dependence may be regulated by serine proteases.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
AB9034-50UL 04053252370571