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MABE670 Anti-Acetyl Histone H3 (Lys27) Antibody, clone 5E2.2

Acetyl Histone H3 (Lys27) Antibody, clone 5E2.2, Cat. No. MABE670, is a highly specific mouse monoclonal antibody, that targets Acetyl Histone H3 (Lys27) and has been tested in Western Blotting and Immunocytochemistry and Dot Blot.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: HIST3H3, H3FT UniProt Number: P61830
MABE670
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HDB, ICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE670
Replaces05-1334
DescriptionAnti-Acetyl Histone H3 (Lys27) Antibody, clone 5E2.2
Alternate Names
  • H3K27Ac
  • Histone H3 (acetyl K27)
  • H3 histone family member T
  • histone cluster 3
  • H3t
Background InformationHistone H3.1t (UniProt: Q16695; also known as H3K27Ac, Histone H3 (acetyl K27), H3 histone family member T, histone cluster 3, H3t) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histones are dynamic proteins that undergo multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone acetylation and deacetylation are reversible and play a major role in determining chromatin structure and function. Histone acetylation occurs mainly on the amino-terminal tail domains of histones. Acetylation neutralizes positive charges on histone and promotes the less condensed and more accessible chromatin conformation known as euchromatin. Histone acetylation is vital in regulating transcriptional activation and DNA replication, recombination, and repair. Histone H3 is one of the five ain histone proteins involved in the structure of chromatin in eukaryotic cells. It is the most extensively modified of the five histones. It features a main globular domain and a long N-terminal tail. Histone H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAcetyl Histone H3 (Lys27) Antibody, clone 5E2.2, Cat. No. MABE670, is a highly specific mouse monoclonal antibody, that targets Acetyl Histone H3 (Lys27) and has been tested in Western Blotting and Immunocytochemistry and Dot Blot.
Key Applications
  • Dot Blot
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Acetyl Histone H3 (Lys27) in Hela cells.



Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected Acetyl Histone H3 (Lys27) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Biological Information
ImmunogenKLH-cojugated peptide corresponding to a sequence around lysine 27 of yeast histone H3.
Clone5E2.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5E2.2 detects histone H3 acetylated on lysine27 in human.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST3H3
  • H3FT
Modifications
  • Acetylation
Purification MethodProtein G purified
UniProt Number
Molecular Weight~17 kDa observed; 15.5 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cells stimulated with Sodium Butyrate.


Western Blotting Analysis: 0.5 µg/mL of this antibody detected Acetyl Histone H3 (Lys27) in 10 µg of HeLa cells stimulated with Sodium Butyrate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
MABE670 04054839156427