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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABN704 Sigma-AldrichAnti-ASS1 Antibody, clone 2C10

Detect Argininosuccinate synthase using this mouse monoclonal antibody, Anti-ASS1 Antibody, clone 2C10 validated for use in western blotting & IHC.

Anti-ASS1 Antibody, clone 2C10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: ASS, CTLN1, ASS1 UniProt Number: P00966

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, M, Mk	WB, IHC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABN704
Description	Anti-ASS1 Antibody, clone 2C10
Alternate Names	Argininosuccinate synthase Citrulline--aspartate ligase ASS1
Background Information	Argininosuccinate synthase (ASS1) or alternatively known by the older name of Citrulline-Aspartate ligase is encoded by the ASS1/ASS gene in humans and is the enzyme that is responsible for the third step in the urea cycle critical to the metabolism of nitrogen. ASS1 catalyzes the synthesis of argininosuccinate from citrulline and aspartate. Argininosuccinate is the immediate precursor of arginine and ASS1 was first identified as the rate limiting step in the liver for this reaction. ASS1 is now recognized to be expressed ubiquitously in mammalian tissues. ASS1 also performs the rate limiting step for NO production in the citrulline-NO cycle as well. Regulation of ASS1 expression can vary depending upon the tissue examined. In the liver, where arginine is hydrolyzed to form urea and ornithine, ASS1 is highly expressed, and various hormones such as glucocorticoids, glucagon, insulin and even high dietary levels of specific amino acids like glutamine regulate expression of ASS1 during development and adult life. In other tissues and in particular in nitric oxide (NO) producing cells, ASS1 is expressed at a low level but induced by proinflammatory cytokines. Interestingly ASS1 expression is also increased in Alzheimer’s disease in both neurons and glia, which also is correlated with iNOS expression and suggests a link between increased NO production and iNOS expression observed in Alzheimer brains. EMD-Millipore’s Anti-ASS1 monoclonal antibody has been tested in western blot on recombinant protein and cell lysates from A431, Raji, MOLT4, Jurkat, A549, NIH3T3, PC-12, and COS7 and in paraffin embedded immunohistochemistry on cervical cancer tissue sections. The antibody has also been tested in direct ELISA on recombinant antigen.

References

Product Information
Format	Purified
Control	A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates
Presentation	Purified mouse monoclonal in PBS with up to 0.1% sodium azide.
Quality Level	MQ100

Applications
Application	Detect Argininosuccinate synthase using this mouse monoclonal antibody, Anti-ASS1 Antibody, clone 2C10 validated for use in western blotting & IHC.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ASS1 in human cervical cancer and colon cancer tissues. Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Purified recombinant fragment of human ASS1 expressed in E. Coli.
Clone	2C10
Host	Mouse
Isotype	IgG1
Species Reactivity	Human Rat Mouse Monkey
Antibody Type	Monoclonal Antibody
Gene Symbol	ASS CTLN1 ASS1
Purification Method	Protein G Purified
UniProt Number	P00966
Molecular Weight	~47 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates. Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ASS1 in A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABN704	04053252918070

Documentation

Anti-ASS1 Antibody, clone 2C10 MSDS

职位
物料安全数据表 (MSDS)

Anti-ASS1 Antibody, clone 2C10 分析证书

标题	批号
Anti-ASS1, clone 2C10 - VP1405163	VP1405163
Anti-ASS1, clone 2C10 - QVP1305301	QVP1305301
Anti-ASS1, clone 2C10 - VP2409057	VP2409057
Anti-ASS1, clone 2C10 -VP1412164	VP1412164
Anti-ASS1, clone 2C10 -VP1507211	VP1507211
Anti-ASS1, clone 2C10 -VP1508201	VP1508201
Anti-ASS1, clone 2C10 -VP1512153	VP1512153
Anti-ASS1, clone 2C10 -VP1603025	VP1603025
Anti-ASS1, clone 2C10 -VP1606071	VP1606071
Anti-ASS1, clone 2C10 Monoclonal Antibody	VP1805310

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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