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MAB348-AF555 Sigma-AldrichAnti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 conjugate

Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 conjugate is an antibody against APP A4 for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
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Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: APP, A4, AD1 UniProt Number: P05067

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
F, Ca, H, M, R, Porcine, Mk	ICC	M	AlexaFluor®555	Monoclonal Antibody

Description
Catalogue Number	MAB348-AF555
Description	Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 conjugate
Alternate Names	Amyloid beta A4 protein ABPP Alzheimer disease amyloid protein Amyloid precursor protein APP APPI Cerebral vascular amyloid peptide CVAP PN-II PreA4 Protease nexin-II PN-II N-APP Soluble APP-alpha S-APP-alpha Soluble APP-beta S-APP-beta C99 Beta-amyloid protein 42 Beta-APP42 Beta-amyloid protein 40 Beta-APP40 C83 P3(42) P3(40) C80 Gamma-secretase C-terminal fragment 59 Amyloid intracellular domain 59 AICD-59 AID(59) Gamma-CTF(59) Gamma-secretase C-terminal fragment 57 Amyloid intracellular domain 57 AICD-57 AID(57) Gamma-CTF(57) Gamma-secretase C-terminal fragment 50 Amyloid intracellular domain 50 AICD-50 AID(50) Gamma-CTF(50) C31
Background Information	Amyloid beta A4 protein (UniProt P05067; also known as ABPP, Alzheimer disease amyloid protein, Amyloid precursor protein, APP, APPI, Cerebral vascular amyloid peptide, CVAP, PN-II, PreA4, Protease nexin-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID 351) in human. Amyloid precursor protein (APP) is initially produced with a signal peptide sequence (a.a. 1-17), the removal of which yields the mature protein with a large extracellular portion (a.a. 18-699), followed by a transmembrane segment (a.a. 700-723) and a cytoplasmic (a.a. 724-770) tail. APP can be further processed by the α-, β-, and γ-secretases in two alternative processing pathways. In the non-amyloidogenic pathway, APP is first cleaved by the plasma membrane-localized α-secretase to generate an N-terminal extracellular sAPPα fragment (a.a. 18-687) and a membrane-bound C-terminal fragment C83 (CTFα), which can be further cleaved by γ-secretase to produce a non-toxic small peptide p3 and a cytoplasmic APP intracellular domain (AICD). In the amyloidogenic pathway, APP undergoes β-cleavage in BACE-1 (β-site APP-cleaving enzyme)-enriched endosomes to generate an N-terminal extracellular sAPPβ fragment (a.a. 18-671) and a membrane-bound C-terminal fragment C99 (CTFβ). Subsequent cleavage of C99 by γ-secretase releases the amyloid β peptide (Aβ) and AICD. Aβ accumulation in the cortical and hippocampal regions of the brain is a major pathological feature of Alzheimer's disease (AD).

References

Product Information
Format	AlexaFluor®555
Presentation	Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality Level	MQ100

Applications
Application	Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 conjugate is an antibody against APP A4 for use in Immunocytochemistry.
Key Applications	Immunocytochemistry
Application Notes	The unconjugated antibody (Cat. No. MAB348) is shown to be suitable also for immunohistochemistry (for both frozen and paraffin-embedded sections), immunofluorescence, and Western blotting applications.

Biological Information
Immunogen	Purified recombinant Alzheimer precursor A4 (pre-A4695) fusion protein.
Epitope	Amino acids 66-81 of APP.
Clone	22C11
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone 22C11 reacts with pre-A4. The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich, C., et al. (1993). J. Biol. Chem. 268(35):26571-26577). Clone 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann, J., et al. (2000). Eur. J. Cell. Biol.79(12):905-914.). The antibody is known to cross react with APLP2 (Slunt, H.H., et al. (1994). J. Biol. Chem. 269(4):2637-2644).
Isotype	IgG1
Species Reactivity	Fish Canine Human Mouse Rat Porcine Monkey
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_201414 NM_000484.2 NM_201413.1
Gene Symbol	APP A4 AD1
Purification Method	Protein A Purfied
UniProt Number	P05067
Molecular Weight	~110/120/130 kDa observed.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Immunocytochemistry in SH-5YSY cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected APP A4 in SH-5YSY cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MAB348-AF555	04055977311976

Documentation

Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 conjugate MSDS

职位
物料安全数据表 (MSDS)

Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 conjugate 分析证书

标题	批号
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 - 3425426	3425426
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 - 4083057	4083057
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 -Q2637233	Q2637233
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 Monoclonal Antibody	2926714
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 555 Monoclonal Antibody	3111121

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标题
Neuroscience Solutions for productive research

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Life Science Research > Antibodies and Assays > Primary Antibodies

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