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ABS31-I-100UG Anti-2A Peptide

Anti-2A Peptide, Cat. No. ABS31-I, is a rabbit polyclonal antibody that detects 2A peptide tagged proteins and is tested for use in Affinity Binding Assay and Western Blotting.

ABS31-I-100UG
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberABS31-I-100UG
DescriptionAnti-2A Peptide
Alternate Names
  • Genome Polyprotein-2A peptide
Background InformationGenome polyprotein (UniProt: P03305) is encoded by the D1Y31_gp1 gene (Gene ID: 37627025) in Foot-and-mouth disease virus. Following its synthesis, it is cleaved into 15 different chains. P2A chain resides in the amino acids 936-953 in the internal region. 2A peptides and 2A-like peptide sequences (known as cis-acting hydrolase elements or CHYSEL) are a superior alternative to internal ribosomal entry sites (IRES) for coordinating the expression of multiple gene products from a single recombinant construct. The 2A sequences are relatively short peptides of about 16-20 amino acids (depending on the virus of origin). They contain a highly conserved sequence, GDVEXNPGP, at the C-terminus, which is shared by different 2As and is essential for the creation of steric hindrance and ribosome skipping. These peptides allow multiple proteins to be encoded as polyproteins, which then dissociate into component proteins upon translation. The 2A sequence impairs normal peptide bond formation via a mechanism called ribosomal skipping, which results in effective, non-enzymatic generation of distinct peptide products from a single multi-cistronic construct. These peptides are used by several families of viruses, the best-known amongst these are foot-and-mouth disease virus 2A (F2A) and Thosea asigna virus 2A (T2A). This rabbit polyclonal antibody reacts with protein in lysate from HEK293T cells transfected with various 2A-tagged proteins. (Ref.: Liu, Z., et al. (2017). Scientific Reports.7; Article number: 2193).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-2A Peptide, Cat. No. ABS31-I, is a rabbit polyclonal antibody that detects 2A peptide tagged proteins and is tested for use in Affinity Binding Assay and Western Blotting.
Key Applications
  • Western Blotting
  • Affinity Binding Assay
Application NotesTested Applications

Affinity Binding Assay: A representative lot of this antibody bound 2A peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids (936-953) 2A peptide of Foot-and-mouth disease virus.
EpitopeInternal
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects 2A peptide tagged proteins.
IsotypeIgG
Species Reactivity
  • All
Species Reactivity NoteAll. Predicted to react with All species.
Antibody TypePolyclonal Antibody
Gene Symbol
  • D1Y31_gp1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight21, 26, and 31 kDa observed; 258.93 kDa for the whole Polyprotein. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HEK293T cells transfected with CD3/g/d/e chains.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected 2A in lysate from HEK293T cells transfected with CD3/g/d/e chains.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABS31-I-100UG 04065267216546