Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

UFC810024 配有Ultracel-100滤膜的Amicon Ultra-4离心式过滤器

UFC810024
24  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Key ApplicationsNMWL体积最低最终浓缩液体积
蛋白提取, 蛋白纯化100 KDa4 mL50 µL
描述
产品目录编号UFC810024
商名
  • Amicon Ultra
描述配有Ultracel-100滤膜的Amicon Ultra-4离心式过滤器
背景信息AMICON ULTRA-4 4ML - 100 KDa cutoff. Available in 3 pack sizes.
UFC810008 - 8 pack
UFC810024 - 24 pack
UFC810096 - 96 pack


High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
High retentate recovery of greater than 90%
Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes)
Heat-sealed membrane minimizes downstream extractables
100% integrity tested for reliable performance
Convenient sample monitoring with translucent housing and volume gradations
Direct pipette sample access eliminates processing step to recover concentrate
High concentration factors of 80–100X

Applications:
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC
Concentration of dilute or pre-purified proteins from column eluents
Desalting, buffer exchange and protein dialysis
参考
产品信息
过滤器代码PLHK
色码透明,带有粉色盖
过滤器类型超滤
NMWL100 KDa
Quality LevelMQ400
应用
应用这款Amicon Ultra 4 mL离心式过滤器借助100 kDa NMWL的滤膜可以进行蛋白浓缩。Amicon Ultra离心式过滤器是蛋白纯化、蛋白浓缩和脱盐的理想工具。
主要应用
  • 蛋白提取
  • 蛋白纯化
应用说明甩平转子:对于稀释样品,离心力为 4,000 x g;对于浓缩后的溶液或粘滞溶液,离心力为 3,500 x g。
固定角度转子:对于 3K、10K、30K 和 50K,离心力为 7,500 x g;对于 100K,离心力为 5,000 x g
生物信息
无菌性非无菌
理化信息
离心应用蛋白浓缩和脱盐
尺寸
长度124 mm (4.88 in.)
直径17.3 mm
过滤面积3 cm²
最低最终浓缩液体积50 µL
体积4 mL
材料信息
化学
  • 再生纤维素(RC)
装置材料
  • 外壳:苯乙烯 / 丁二烯;管子:聚丙烯;盖子和衬里:聚乙烯
支撑材料Regenerated cellulose
毒性学信息
GHS安全信息
安全信息
产品使用声明
储存和货运信息
包装信息
数量24
运输信息
补充信息
规格
Global Trade Item Number
产品目录编号 GTIN
UFC810024 04053252666377

Documentation

参考

参考概述应用
Modification of Allergenicity and immunogenicity of formate dehydrogenase by conjugation with linear mono methoxy poly ethylene glycol: Improvement in detoxification of formate in methanol poisoning
Muthuvel A., Rajamani R., Senthilvelan M., Manikandan s., Sheeladevi R.
Clinica Chimica acta 374 (2006) 122-128  2006

Protein Purification

小册子

标题
REFINE PROTEIN PREPARATION. Tools for better protein analysis

用户指南

标题
Amicon® Ultra-4 Centrifugal Filter Devices Non IVD User guide