Amicon Ultra-15离心过滤器
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概述
规格
订货信息
Documentation
参考
| 参考概述 | 应用 |
|---|---|
| Simultaneous reduction and alkylation of protein disulfides in a centrifugal ultrafiltration device prior to two-dimensional gel electrophoresis. Smejkal GB, Li C, Robinson MH, Lazarev AV, Lawrence NP, Chernokalskaya E. J Proteome Res. 2006 Apr;5(4):983-7. 2006 | Protein Concentration |
| Ultrafiltration for proteomic sample preparation E. Chernokalskaya, S. Gutierrez, A.M. Pit, J. T. Leonard Electrophoresis, 25: 2461-2468 2004 | Sample Prep |
问与答
| 问题 | 回答 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amicon Ultra units are offered in what volumes? | Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes. | ||||||||||||
| What is the recommended rotor for Amicon Ultra units? | Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units. | ||||||||||||
| How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra? | Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest. | ||||||||||||
| Which membranes are available in Amicon Ultra units? | Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO. | ||||||||||||
| Can the Amicon Ultra be spun to dryness? | No, the Amicon Ultra has a built in deadstop. | ||||||||||||
| Can the Amicon Ultra be used with nucleic acids? | Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide. | ||||||||||||
| What is the maximum sample volume for Amicon Ultra? |
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| What is the maximum centrifugal force I can spin Amicon Ultra-4 & Amicon Ultra-15? |
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| What is the chemical compatibility of Amicon Ultra? | Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support. | ||||||||||||
| Can Amicon Ultra units be sterilized with ethanol? | Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization. |
相关产品和应用
相关产品，按下列排序： Application Facete
| Protein Purification |
| Dialysis and Filtration |
相关产品，按下列排序： Brand Facete
| Ultracel® |
| Amicon® |
种类
| Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Sample Prep Centrifugal Filter Units |
- 高回收率再生纤维素滤膜有多种分子量截止值可选
- 截留回收率高达>90%
- 竖直滤膜避免了超快旋转时间（速度达10-15分钟）时的浓度极化
- 热密封滤膜最大程度地减少了下游提取物
- 100%经过完整性测试，性能可靠
- 透明外壳和体积刻度便于监测样品
- 具有用移液管直接吸取样品的开口，省却了恢复浓度的处理步骤
- 具有80–100X大浓缩因子
应用
- 浓缩含有抗原、抗体、酶、核酸或微生物的生物样品
- 纯化组织培养提取液或细胞裂解液中的大分子成分，在进行HPLS之前去除蛋白
- 浓缩柱洗脱液的稀释或预纯化蛋白
- 脱盐、缓冲液置换和蛋白透析
不确定是否为您的应用找到了合适的Amicon？
» 请用我们的新型Amicon Ultra选择器工具搜索。
滤液体积所对应的典型旋转时间
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离心条件：4000 x g 吊篮式转子 (25°C)，15 mL 样品。3K，10K：细胞色素 c (0.25 mg/mL)；30K，50K：BSA (1 mg/mL)；100K：IgG (1 mg/mL)。
典型的蛋白回收率
| 滤膜NMWL，kDa | 蛋白溶质 | 蛋白截留物体积，mL | 蛋白截留物回收率，% |
| 3 | 细胞色素c，12.4 kDa (0.25 mg/mL) | 0.294 | 97.5 |
| 10 | 细胞色素c，12.4 kDa (0.25 mg/mL) | 0.24 | 93.03 |
| 30 | BSA，67 kDa (1 mg/mL) | 0.30 | 97.94 |
| 50 | BSA，67 kDa (1 mg/mL) | 0.17 | 93.32 |
| 100 | IgG，156 kDa (1 mg/mL) | 0.28 | 89.03 |
| 4000 x g、25 °C下摆桶电机中15 mL起始体积 旋转时间：3K (40 min)；10K和100K (20 min)；30K (10 min)；50K (15 min)。 | |||
| 体积 | |
| 最大初始样品体积，mL* | 15 mL |
| 典型最终浓缩体积，µL | 200 µL |
| 离心力 | 甩平转子：4,000 x g； 固定角度转子 (35°)：5,000 x g |
| 材料 | |
| 滤膜 | Ultracel 再生纤维素 |
| 装置 | 外壳：苯乙烯 / 丁二烯；管子：聚丙烯；盖子和衬里：聚乙烯 |
| 尺寸 | |
| 有效滤膜面积 | 7.6 cm² |
| 直径 | 2.97 cm |
| 滤液管或小杯（离心管） | |
| 长度 | 12.1 cm (4.807 in.) |
| 过滤器 | |
| 直径，mm | 29.6 |
| 长度，mm | 72.0 |
| *35° 定角马达除外：12 mL | |
配有Ultracel-10滤膜的Amicon Ultra-15离心式过滤器已获得CE认证。