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UFC910096 配有Ultracel-100滤膜的Amicon Ultra-15离心式过滤器

UFC910096
96  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Key ApplicationsNMWL体积最低最终浓缩液体积
蛋白提取, 蛋白纯化100 KDa15 mL200 µL
描述
产品目录编号UFC910096
商名
  • Amicon Ultra
描述配有Ultracel-100滤膜的Amicon Ultra-15离心式过滤器
背景信息AMICON ULTRA-15 15ML - 100 KDa cutoff. Available in 3 pack sizes.
UFC910008 - 8 pack
UFC910024 - 24 pack
UFC910096 - 96 pack



High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
High retentate recovery of greater than 90%
Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
Heat-sealed membrane minimizes downstream extractables
100% integrity tested for reliable performance
Convenient sample monitoring with translucent housing and volume gradations
Direct pipettor sample access eliminates processing step to recover concentrate
High concentration factors of 80–100X

Applications:
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
Concentration of dilute or pre-purified proteins from column eluents
Desalting, buffer exchange and protein dialysis
参考
产品信息
HS代码8421 29 90
过滤器代码PLHK
色码透明,带有粉色盖
过滤器类型超滤
NMWL100 KDa
Quality LevelMQ400
应用
应用这款Amicon Ultra 15 mL离心式过滤器借助100 kDa NMWL的滤膜可以进行蛋白浓缩。Amicon Ultra离心式过滤器是蛋白纯化、蛋白浓缩和脱盐的理想工具。
主要应用
  • 蛋白提取
  • 蛋白纯化
应用说明甩平转子:4,000 x g;
固定角度转子 (35°):5,000 x g
生物信息
无菌性非无菌
理化信息
离心应用蛋白浓缩和脱盐
尺寸
长度12.1 cm (4.807 in.)
直径2.97 cm
过滤面积7.6 cm²
最低最终浓缩液体积200 µL
体积15 mL
材料信息
化学
  • 再生纤维素(RC)
装置材料
  • 外壳:苯乙烯 / 丁二烯;管子:聚丙烯;盖子和衬里:聚乙烯
支撑材料Regenerated cellulose
毒性学信息
GHS安全信息
安全信息
产品使用声明
储存和货运信息
包装信息
数量96
运输信息
补充信息
规格
Global Trade Item Number
产品目录编号 GTIN
UFC910096 04053252316517

Documentation

小册子

标题
REFINE PROTEIN PREPARATION. Tools for better protein analysis

问与答

问题回答
Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
Which membranes are available in Amicon Ultra units?Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
What is the maximum sample volume for Amicon Ultra?
Amicon Ultra-4Amicon Ultra-15
Swinging Bucket4ml 15ml
Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
3.5ml(23 degree rotor angle)
What is the maximum centrifugal force I can spin Amicon Ultra-4 & Amicon Ultra-15?
4ml15ml
Swinging Bucket4000 x g4000 x g
3500 x g for concentrated/viscous solutions
Fixed Angle(23, 35 &45 degrees)7500 x g5000 x g
What is the chemical compatibility of Amicon Ultra?Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support.
Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.

用户指南

标题
Amicon® Ultra-15 Centrifugal Filter Devices Non IVD User guide