Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

UFC910024 Amicon Ultra-15，PLHK Ultracel-PL 超滤膜，100 kDa

Ultracel-100 regenerated cellulose membrane, 15 mL sample volume

Amicon Ultra-15，PLHK Ultracel-PL 超滤膜，100 kDa MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
主要应用: 蛋白提取, 蛋白纯化
UFC910024
24  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Key ApplicationsNMWL体积最低最终浓缩液体积
蛋白提取, 蛋白纯化100 KDa15 mL200 µL
描述
产品目录编号UFC910024
商名
  • Amicon Ultra
描述Amicon Ultra-15，PLHK Ultracel-PL 超滤膜，100 kDa
背景信息AMICON ULTRA-15 15ML - 100 KDa cutoff. Available in 3 pack sizes.
UFC910008 - 8 pack
UFC910024 - 24 pack
UFC910096 - 96 pack



High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
High retentate recovery of greater than 90%
Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
Heat-sealed membrane minimizes downstream extractables
100% integrity tested for reliable performance
Convenient sample monitoring with translucent housing and volume gradations
Direct pipettor sample access eliminates processing step to recover concentrate
High concentration factors of 80–100X

Applications:
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
Concentration of dilute or pre-purified proteins from column eluents
Desalting, buffer exchange and protein dialysis
参考
产品信息
过滤器代码PLHK
色码透明，带有粉色盖
过滤器类型超滤
NMWL100 KDa
Quality LevelMQ400
应用
应用Ultracel-100 regenerated cellulose membrane, 15 mL sample volume
主要应用
  • 蛋白提取
  • 蛋白纯化
应用说明甩平转子：4,000 x g；
固定角度转子 (35°)：5,000 x g
生物信息
无菌性非无菌
理化信息
离心应用蛋白浓缩和脱盐
尺寸
长度12.1 cm (4.807 in.)
直径2.97 cm
过滤面积7.6 cm²
最低最终浓缩液体积200 µL
体积15 mL
材料信息
化学
  • 再生纤维素（RC）
装置材料
  • 离心管：聚丙烯
  • 离心管盖和封口：聚乙烯
支撑材料Regenerated cellulose
毒性学信息
GHS安全信息
安全信息
产品使用声明
储存和货运信息
包装信息
数量24
运输信息
补充信息
规格
Global Trade Item Number
产品目录编号 GTIN
UFC910024 04053252317439

Documentation

参考

参考概述公共医疗ID
Specific Lipids Modulate the Transporter Associated with Antigen Processing (TAP).
Schölz, Christian, et al.
J. Biol. Chem., 286: 13346-56 (2011)  2011

显示摘要 		21357424 21357424

质量证书

标题
UFC910024 - 0000241948

小册子

标题
REFINE PROTEIN PREPARATION. Tools for better protein analysis

问与答

问题回答
Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
Which membranes are available in Amicon Ultra units?Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
What is the maximum sample volume for Amicon Ultra?
Amicon Ultra-4Amicon Ultra-15
Swinging Bucket4ml 15ml
Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
3.5ml(23 degree rotor angle)
What is the chemical compatibility of Amicon Ultra?Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support.
Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.
Do you have any centrifugal filter units that are pyrogen free?We do not have any centrifugals devices that we claim are pyrogen free. Even the sterile devices could contain pyrogens.
Show more

用户指南

标题
Amicon® Ultra-15 Centrifugal Filter Devices Non IVD User guide