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14-840M AMPK (α1, β1, γ1) Protein, active, 250 µg

Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

AMPK (α1, β1, γ1) Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-840M
250 µg  Also available in 10 μg size (cat#14-840) and in bulk (cat# 14-840-K).
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-840M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAMPK (α1, β1, γ1) Protein, active, 250 µg
OverviewRecombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 and full length untagged AMPKγ1.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed in E. coli cells and activated in vivo.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene Symbol
  • PRKAB1
  • PRKAG1
  • PRKAA1
Protein TargetAMPK (α1, β1, γ1)
Purification MethodCobalt-agarose
Target Sub-FamilyCAMK
UniProt Number
Molecular WeightAMPKα1 MW = 64 kDa, AMPKß1 MW = 30 kDa, AMPKγ1 MW = 38 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-840) and in bulk (cat# 14-840-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-840M 04053252739705

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产品目录编号 描述
14-840 AMPK (α1, β1, γ1) Protein, active, 10 µg

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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases