| Column | Chromolith® Performance RP-18e 100-3 mm |
|---|---|
| Mobile Phase | Acetonitrile / buffer pH 1.8 (gradient) |
| Flow rate | 2.0 mL/min |
| Pressure | 92 bar |
| Detection | UV 254 nm |
| Temp. | 30°C |
| Injection volume | 1 μL |
| Sample | 1. - 2. By-products 3. Levothyroxine 4. - 9. By-products> |
 |
|
Chromolith® RP-18 Endcapped HPLC Columns |
Fast Separation at Lower Flow Rates
Chromolith Performance RP-18 endcapped HPLC columns are designed for rapid separations of more complex mixtures. The Chromolith® 3 mm internal-diameter column (length 100 mm) is an ideal alternative to conventional particulate columns with internal diameters of 4.6, 4 or 3 mm. Even difficult separations, which often take 15 to 30 minutes on particulate columns, typically take only 5 to 10 minutes on Chromolith® 3 mm i.d.
Column Coupling
Chromolith® 3 mm i.d. columns can be easily coupled using the column coupler to form columns of 20 cm or longer as required. As shown in Tables 1 and 2, this results in very high peak resolution at moderate pressure with flow rates between 1 and 1.5 mL/min.
Excellent Column Efficiency and Peak Resolution
Table 3 depicts a typical fast separation of four compounds in less than two minutes using a Chromolith® 4.6 mm i.d. column at a flow rate of 4 mL/min. The second example (Table 4) shows the same separation on a Chromolith® 3 mm i.d. column. Both chromatograms exhibit excellent column efficiency and peak resolution, however the 3 mm i.d. column demonstrates improved sensitivity at just 1.7 mL/min, thus saving 57% of solvents.
Chromolith® RP-18 Endcapped 2 mm and 3mm Columns
Product |
Internal Diameter |
Ordering Number |
|---|---|---|
| Chromolith® Performance RP-18 endcapped | 100 x 2 mm 100 x 3 mm |
152006 152001 |
| Chromolith® Flash RP-18 endcapped | 25 x 2 mm 25 x 3 mm |
152014 152003 |
| Chromolith® FastGradient RP-18 endcapped | 50 x 2 mm 50 x 3 mm |
152007 152002 |
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