Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

69909 pET-30a(+) DNA - Novagen

Novagen's pET-30a-c(+) vectors carry an N-terminal His•Tag®/thrombin/S•Tag™/enterokinase configuration plus an optional C-terminal His•Tag sequence.

pET-30a(+) DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69909
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69909-3CN Glass bottle 10 μg
Description
OverviewThe pET-30a-c(+) vectors carry an N-terminal His•Tag®/thrombin/S•Tag™/enterokinase configuration plus an optional C-terminal His•Tag sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB095).

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69909
Brand Family Novagen®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69909-3CN 04055977258783

Documentation

pET-30a(+) DNA - Novagen SDS

Title

Safety Data Sheet (SDS) 

pET-30a(+) DNA - Novagen Certificates of Analysis

TitleLot Number
69909

Citations

Title
  • Sarah A. Clock, et al. (2008) Outer membrane components of the Tad (tight adherence) secreton of Aggregatibacter actinomycetemcomitans. Journal of Bacteriology 190, 980-990.
  • Jeremy H. Herskowitz, et al. (2008) Systematic mutagenesis of the murine gammaherpesvirus 68 M2 protein identifies domains important for chronic infection. Journal of Virology 82, 3295-3310.
  • Anthony Argentaro, et al. (2007) Structural consequences of disease-causing mutations in the ATRX-DNMT3-DNMT3L (ADD) domain of the chromatin-associated protein ATRX. Procedings of the National Academy of Science 104, 11939-11944.
  • Srividya Balasubramanian, Sami R. Fam and Randy A. Hall. (2007) GABAB receptor association with the PDZ scaffold mupp1 alters receptor stability and function. Journal of Biological Chemistry 282, 4162-4171.
  • Meng Qi, Hyun-Sik Jun and Cecil W. Forsberg. (2007) Characterization and synergistic interactions of Fibrobacter succinogenes glycoside hydrolases. Applied and Enviornmental Microbiology 73, 6098-6105.
  • Satoshi Tasumi and Gerardo R. Vasta. (2007) A galectin of unique domain organization from hemocytes of the eastern oyster (Crassostrea virginica) is a receptor for the protistan parasite Perkinsus marinus. Journal of Immunology 179, 3086-3098.
  • Kai-Hong Zhao, et al. (2007) Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins. Procedings of the National Academy of Science 104, 14300-14305.
  • Chongguang Chen, et al. (2006) GEC1 interacts with the opioid receptor and enhances expression of the receptor. Journal of Biological Chemistry 281, 7983-7993.
  • Dmitri Nashchekin, et al. (2006) A novel ded1-like RNA helicase interacts with the Y-box protein ctYB-1 in nuclear mRNP particles and in polysomes. Journal of Biological Chemistry 281, 14263-14272.
  • Jiping Xiao, et al. (2006) Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis. Journal of Biological Chemistry 281, 15182-15193.
  • Kai-Hong Zhao, et al. (2006) Chromophore attachment to phycobiliprotein -subunits: phycocyanobilin:cysteine-84 phycobiliprotein lyase activity of Cpes-like protein from Anabaena SP. PCC7120. Journal of Biological Chemistry 281, 8573-8581.
  • Min-Hsuan Chen, et al. (2005) Phospholipid scramblase 1 (PLSCR1) contains a non-classical nuclear localization signal with unique binding site in importin a. Journal of Biological Chemistry 280, 10599-10606.
  • Erika Crouch, et al. (2005) Ligand specificity of human surfactant protein D: expression of a mutant trimeris collection that shows enhanced interactions with influenza A virus. Journal of Biological Chemistry 280, 17046-17056.
  • Neelam Desai, et al. (2005) Two steps in Maf1 dependent repression of transcription by RNA Polymerase III. Journal of Biological Chemistry 280, 6455-6462.
  • Jong-Min Han, et al. (2005) Structural and functional properties of V156K and A158E mutants of apolipoprotein A-I in the lipid-free and lipid-bound state. Journal of Lipid Research 46, 589-596.
  • J. K. Jang, T. Rahman and K. S. McKim. (2005) The kinesinlike protein subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. 16, 4684-4694.
  • Aki Jarvinen, et al. (2005) Metabolic stability of alpha -methylated polyamine derivatives and their use as substitutes for the natural polyamines. Journal of Biological Chemistry 280, 6595-6601.
  • Torsten Kessler, et al. (2005) Inhibition of tumor growth by RGD peptide-directed delivery of truncated tissue factor to the tumor vasculature. Clinical Cancer Research 11, 6317-6324.
  • Alexei Kharitonenkov, et al. (2005) FGF-21 as a novel metabolic regulator. Journal of Clinical Investigation 115, 1627-1635.
  • Jui-Ming Lin, Analyne Schroeder and Ravi Allada. (2005) In vivo circadian function of casein kinase 2 phosphorylation sites in Drosophila PERIOD. Journal of Neuroscience 25, 11175-11183.
  • Catherine Paradis-Bleau, Francois Sanschagrin and Roger C. Levesque. (2005) Peptide inhibitors of the essential cell division protein FtsA. Protein Engineering Design and Selection 18, 85-91.
  • Daniel Picard, C. Cheng Kao and Katalin A. Hudak. (2005) Pokeweed antiviral protein inhibits brome mosaic virus replication in plant cells. Journal of Biological Chemistry 280, 20069-20075.
  • Alexander J. Ruthenburg, et al. (2005) A superhelical spiral in the Escherichia coli DNA gyrase A C-terminal domain imparts unidirectional supercoiling bias. Journal of Biological Chemistry 280, 26177-26184.
  • Francois Sanschagrin and Roger C. Levesque. (2005) A specific peptide inhibitor of the class B metallo-{beta}-lactamase L-1 from Stenotrophomonas maltophilia identified using phage display. Journal of Antimicrobial Chemotherapy 55, 252-255.
  • Tien-Shin Yu, et al. (2005) α-Amylase is not required for breakdown of transitory starch in Arabidopsis leaves. Journal of Biological Chemistry 280, 9773-9779.
  • Deliang Guo, et al. (2004) Identification and characterization of a novel cytoplasm protein ICF45 that is involved in cell cycle regulation. Journal of Biological Chemistry 279, 53498-53505.
  • Ai Xiao Liu, et al. (2004) Soluble expression and characterization of a GFP-fused pea actin isoform (PEAc1). Cell Research 14, 407-414.
  • Laura Morelli, et al. (2004) Insulin-degrading enzyme in brain microvessels: proteolysis of amyloid vasculotropic variants and reduced activity in cerebral amyloid angiopathy. Journal of Biological Chemistry 279, 56004-56013.
  • Cynthia L. Richard, Animesh Tandon and Robert G. Kranz. (2004) Rhodobacter capsulatus nifA1 promoter: high-GC -10 regions in High-GC bacteria and the basis for their transcription. Journal of Bacteriology 186, 740-749.
  • R.P. Hirt, et al. (2003) Biochemical and genetic evidence for a family of heterotrimeric G-proteins in Trichomonas vaginalis. Molecular and Biochemical Parasitology 129, 179-189.
  • Ramin Nazarian, Juan M. Falcon-Perez and Esteban C. Dell'Angelica. (2003) Biogenesis of lysozyme-related organelles complex 3 (BLOC-3): a complex containing the Hermansky- Pudlak syndrome (HPS) proteins HPS1 and HPS4. Procedings of the National Academy of Science 100, 8770-8775.
  • Ahmed El Zoeiby, et al. (2003) Identification of novel inhibitors of Pseudomonas aeruginosa MurC enzyme derived from phage-displayed peptide libraries. Journal of Antimicrobial Chemotherapy 51, 531-543.
  • Jui-Ming Lin, et al. (2002) A role for casein kinase 2 α in the Drosophila circadian clock. 420, 816-820.
  • Bryony A.P. Williams, et al. (2002) A mitochondrial remnant in the microsporidian Trachipleistophora hominis. 418, 865-869.
  • Robert H. Baloh, et al. (1998) Artemin, a novel member of the GDNF ligand family, supports perephial and central neurons and signals through the GFRα3-RET receptor complex. Neuron 21, 1291-1302.
  • R.A. Hall, et al. (1998) The β2-adrenergic receptor interacts with the Na+/H+ exchange. 392, 626-630.
  • Douglas J. Creedon, et al. (1997) Neurturin shares receptors and signal transduction pathways with glial cell line-derived neurotrophic factor in sympathetic neurons. Procedings of the National Academy of Science 94, 7018-7023.
  • L.del Peso, et al. (1997) Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt. Science 278, 687-689.
  • H. Uchida, J. Zhang and D.S.D. Nimer. (1997) AML1A and AML1B can transactivate the human IL-3 promoter. Journal of Immunology 158, 2251-2258.
    		•

    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB095 pET-30a-c(+) Vector Map

    Related Products & Applications

    Related Products

    Catalogue Number Description
    69418 pET-3a DNA - Novagen
    69419 pET-3b DNA - Novagen
    69421 pET-3d DNA - Novagen
    69431 pET-9a DNA - Novagen
    69434 pET-9d DNA - Novagen
    69436 pET-11a DNA - Novagen
    69439 pET-11d DNA
    69659 pLysS DNA - Novagen
    69660 pET-14b DNA - Novagen
    69661 pET-15b DNA - Novagen
    Show more

    Categories

    Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors